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[[Image:1k1a.gif|left|200px]]


{{Structure
==Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family==
|PDB= 1k1a |SIZE=350|CAPTION= <scene name='initialview01'>1k1a</scene>, resolution 1.86&Aring;
<StructureSection load='1k1a' size='340' side='right'caption='[[1k1a]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1k1a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K1A FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k1a OCA], [https://pdbe.org/1k1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k1a RCSB], [https://www.ebi.ac.uk/pdbsum/1k1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k1a ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[1k1b|1K1B]]
== Disease ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k1a OCA], [http://www.ebi.ac.uk/pdbsum/1k1a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k1a RCSB]</span>
[https://www.uniprot.org/uniprot/BCL3_HUMAN BCL3_HUMAN] Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
}}
== Function ==
[https://www.uniprot.org/uniprot/BCL3_HUMAN BCL3_HUMAN] Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).<ref>PMID:8453667</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k1/1k1a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k1a ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
IkappaB proteins associate with the transcription factor NF-kappaB via their ankyrin repeat domain. Bcl-3 is an unusual IkappaB protein because it is primarily nucleoplasmic and can lead to enhanced NF-kappaB-dependent transcription, unlike the prototypical IkappaB protein IkappaBalpha, which inhibits NF-kappaB activity by retaining it in the cytoplasm. Here we report the 1.9 A crystal structure of the ankyrin repeat domain of human Bcl-3 and compare it with that of IkappaBalpha bound to NF-kappaB. The two structures are highly similar over the central ankyrin repeats but differ in the N-terminal repeat and at the C-terminus, where Bcl-3 contains a seventh repeat in place of the acidic PEST region of IkappaBalpha. Differences between the two structures suggest why Bcl-3 differs from IkappaBalpha in selectivity towards various NF-kappaB species, why Bcl-3 but not IkappaBalpha can associate with its NF-kappaB partner bound to DNA, and why two molecules of Bcl-3 but only one of IkappaBalpha can bind to its NF-kappaB partner. Comparison of the two structures thus provides an insight into the functional diversity of IkappaB proteins.


'''Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family'''
Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family.,Michel F, Soler-Lopez M, Petosa C, Cramer P, Siebenlist U, Muller CW EMBO J. 2001 Nov 15;20(22):6180-90. PMID:11707390<ref>PMID:11707390</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1k1a" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
IkappaB proteins associate with the transcription factor NF-kappaB via their ankyrin repeat domain. Bcl-3 is an unusual IkappaB protein because it is primarily nucleoplasmic and can lead to enhanced NF-kappaB-dependent transcription, unlike the prototypical IkappaB protein IkappaBalpha, which inhibits NF-kappaB activity by retaining it in the cytoplasm. Here we report the 1.9 A crystal structure of the ankyrin repeat domain of human Bcl-3 and compare it with that of IkappaBalpha bound to NF-kappaB. The two structures are highly similar over the central ankyrin repeats but differ in the N-terminal repeat and at the C-terminus, where Bcl-3 contains a seventh repeat in place of the acidic PEST region of IkappaBalpha. Differences between the two structures suggest why Bcl-3 differs from IkappaBalpha in selectivity towards various NF-kappaB species, why Bcl-3 but not IkappaBalpha can associate with its NF-kappaB partner bound to DNA, and why two molecules of Bcl-3 but only one of IkappaBalpha can bind to its NF-kappaB partner. Comparison of the two structures thus provides an insight into the functional diversity of IkappaB proteins.
*[[B-cell lymphoma proteins 3D structures|B-cell lymphoma proteins 3D structures]]
 
== References ==
==About this Structure==
<references/>
1K1A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K1A OCA].
__TOC__
 
</StructureSection>
==Reference==
Crystal structure of the ankyrin repeat domain of Bcl-3: a unique member of the IkappaB protein family., Michel F, Soler-Lopez M, Petosa C, Cramer P, Siebenlist U, Muller CW, EMBO J. 2001 Nov 15;20(22):6180-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11707390 11707390]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cramer, P.]]
[[Category: Cramer P]]
[[Category: Michel, F.]]
[[Category: Michel F]]
[[Category: Mueller, C W.]]
[[Category: Mueller CW]]
[[Category: Petosa, C.]]
[[Category: Petosa C]]
[[Category: Siebenlist, U.]]
[[Category: Siebenlist U]]
[[Category: Soler-Lopez, M.]]
[[Category: Soler-Lopez M]]
[[Category: bcl-3]]
[[Category: ikappab protein]]
[[Category: nf-kappab transcription factor]]
 
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Retrieved from "https://proteopedia.org/1k1a"