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[[Image:1k43.gif|left|200px]]


{{Structure
==10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)==
|PDB= 1k43 |SIZE=350|CAPTION= <scene name='initialview01'>1k43</scene>
<StructureSection load='1k43' size='340' side='right'caption='[[1k43]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1k43]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K43 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k43 OCA], [https://pdbe.org/1k43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k43 RCSB], [https://www.ebi.ac.uk/pdbsum/1k43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k43 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[1j4m|1J4M]]
<div style="background-color:#fffaf0;">
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k43 OCA], [http://www.ebi.ac.uk/pdbsum/1k43 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k43 RCSB]</span>
== Publication Abstract from PubMed ==
}}
 
'''10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)'''
 
 
==Overview==
Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.
Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.


==About this Structure==
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides.,Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:11782528<ref>PMID:11782528</ref>
1K43 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K43 OCA].
 
==Reference==
Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides., Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E, Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11782528 11782528]
[[Category: Protein complex]]
[[Category: Lacroix, E.]]
[[Category: Pastor, M T.]]
[[Category: Paz, M Lopez de la.]]
[[Category: Perez-Paya, E.]]
[[Category: Serrano, L.]]
[[Category: beta-hairpin]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:43:07 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1k43" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lacroix E]]
[[Category: Lopez de la Paz M]]
[[Category: Pastor MT]]
[[Category: Perez-Paya E]]
[[Category: Serrano L]]
Retrieved from "https://proteopedia.org/1k43"