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==3.5 A structure of fragment I from E. coli 5S RRNA==
==3.5 A structure of fragment I from E. coli 5S RRNA==
<StructureSection load='357d' size='340' side='right' caption='[[357d]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
<StructureSection load='357d' size='340' side='right'caption='[[357d]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[357d]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=357D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=357D FirstGlance]. <br>
<table><tr><td colspan='2'>[[357d]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=357D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=357D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SSU:URIDINE-5-PHOSPHOROTHIOATE'>SSU</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SSU:URIDINE-5-PHOSPHOROTHIOATE'>SSU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=357d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=357d OCA], [http://pdbe.org/357d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=357d RCSB], [http://www.ebi.ac.uk/pdbsum/357d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=357d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=357d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=357d OCA], [https://pdbe.org/357d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=357d RCSB], [https://www.ebi.ac.uk/pdbsum/357d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=357d ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Two new RNA structures portray how non-Watson-Crick base pairs and metal ions can produce a unique RNA shape suitable for recognition by proteins. The crystal structures of a 62 nt domain of E. coli 5S ribosomal RNA and a duplex dodecamer encompassing an internal loop E have been determined at 3.0 and 1.5 A, respectively. This loop E region is distorted by three "cross-strand purine stacks" and three novel, water-mediated noncanonical base pairs and stabilized by a four metal ion zipper. These features give its minor groove a unique hydrogen-bonding surface and make the adjacent major groove wide enough to permit recognition by the ribosomal protein L25, which is expected to bind to this surface.
Metals, motifs, and recognition in the crystal structure of a 5S rRNA domain.,Correll CC, Freeborn B, Moore PB, Steitz TA Cell. 1997 Nov 28;91(5):705-12. PMID:9393863<ref>PMID:9393863</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 357d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Correll, C C]]
[[Category: Escherichia coli]]
[[Category: Freeborn, B]]
[[Category: Large Structures]]
[[Category: Moore, P B]]
[[Category: Correll CC]]
[[Category: Steitz, T A]]
[[Category: Freeborn B]]
[[Category: Double helix]]
[[Category: Moore PB]]
[[Category: Internal loop]]
[[Category: Steitz TA]]
[[Category: Kinked]]
[[Category: Mismatched]]
[[Category: Modified]]
[[Category: Overhanging base]]
[[Category: Rna]]
[[Category: U-rna]]

Latest revision as of 08:39, 7 February 2024

3.5 A structure of fragment I from E. coli 5S RRNA

357d, resolution 3.50Å

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