|
|
| (One intermediate revision by the same user not shown) |
| Line 1: |
Line 1: |
|
| |
|
| ==Crystal structure of human LGR5-RSPO1-RNF43== | | ==Crystal structure of human LGR5-RSPO1-RNF43== |
| <StructureSection load='4kng' size='340' side='right' caption='[[4kng]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='4kng' size='340' side='right'caption='[[4kng]], [[Resolution|resolution]] 2.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4kng]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KNG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KNG FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4kng]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KNG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KNG FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LGR5, GPR49, GPR67 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), RSPO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), RNF43 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kng OCA], [http://pdbe.org/4kng PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kng RCSB], [http://www.ebi.ac.uk/pdbsum/4kng PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kng ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kng OCA], [https://pdbe.org/4kng PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kng RCSB], [https://www.ebi.ac.uk/pdbsum/4kng PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kng ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Disease ==
| |
| [[http://www.uniprot.org/uniprot/RSPO1_HUMAN RSPO1_HUMAN]] Palmoplantar keratoderma - XX sex reversal - predisposition to squamous cell carcinoma. Keratoderma, palmoplantar, with squamous cell carcinoma of skin and sex reversal (PKKSCC) [MIM:[http://omim.org/entry/610644 610644]]: A recessive syndrome characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Note=The disease is caused by mutations affecting the gene represented in this entry.
| |
| == Function == | | == Function == |
| [[http://www.uniprot.org/uniprot/LGR5_HUMAN LGR5_HUMAN]] Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. [[http://www.uniprot.org/uniprot/RNF43_HUMAN RNF43_HUMAN]] E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.<ref>PMID:18313049</ref> <ref>PMID:22575959</ref> <ref>PMID:22895187</ref> [[http://www.uniprot.org/uniprot/RSPO1_HUMAN RSPO1_HUMAN]] Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.<ref>PMID:16109882</ref> <ref>PMID:22575959</ref> | | [https://www.uniprot.org/uniprot/LGR5_HUMAN LGR5_HUMAN] Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 21: |
|
| |
|
| ==See Also== | | ==See Also== |
| *[[Ubiquitin protein ligase|Ubiquitin protein ligase]] | | *[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]] |
| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Human]] | | [[Category: Homo sapiens]] |
| [[Category: Chen, P H]] | | [[Category: Large Structures]] |
| [[Category: He, X]] | | [[Category: Chen PH]] |
| [[Category: Cysteine-rich domain]] | | [[Category: He X]] |
| [[Category: Furin-repeat]]
| |
| [[Category: Leucine-rich repeat]]
| |
| [[Category: Ligand recognition]]
| |
| [[Category: Membrane protein]]
| |
| [[Category: N-linked glycosylation]]
| |
| [[Category: Protease-associated domain]]
| |
| [[Category: Protein-protein interaction]]
| |
| [[Category: Signaling protein]]
| |
