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| [[Image:1lvq.gif|left|200px]]
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| {{Structure
| | ==IC3 of CB1 Bound to G(alpha)i== |
| |PDB= 1lvq |SIZE=350|CAPTION= <scene name='initialview01'>1lvq</scene>
| | <StructureSection load='1lvq' size='340' side='right'caption='[[1lvq]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1lvq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVQ FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvq OCA], [https://pdbe.org/1lvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvq RCSB], [https://www.ebi.ac.uk/pdbsum/1lvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvq ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=[[ilvr|ILVR]]
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvq OCA], [http://www.ebi.ac.uk/pdbsum/1lvq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lvq RCSB]</span>
| | [https://www.uniprot.org/uniprot/CNR1_HUMAN CNR1_HUMAN] Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.<ref>PMID:15620723</ref> |
| }}
| | == References == |
| | | <references/> |
| '''IC3 of CB1 Bound to G(alpha)i'''
| | __TOC__ |
| | | </StructureSection> |
| | | [[Category: Homo sapiens]] |
| ==Overview==
| | [[Category: Large Structures]] |
| The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.
| | [[Category: Kendall DA]] |
| | | [[Category: McMurry JL]] |
| ==About this Structure==
| | [[Category: Mierke DF]] |
| 1LVQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVQ OCA].
| | [[Category: Miller A]] |
| | | [[Category: Ulfers AL]] |
| ==Reference== | | [[Category: Wang L]] |
| Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity., Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF, Protein Sci. 2002 Oct;11(10):2526-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12237474 12237474]
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| [[Category: Single protein]] | |
| [[Category: Kendall, D A.]] | |
| [[Category: McMurry, J L.]] | |
| [[Category: Mierke, D F.]] | |
| [[Category: Miller, A.]] | |
| [[Category: Ulfers, A L.]] | |
| [[Category: Wang, L.]] | |
| [[Category: alpha domain of g protein i]]
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| [[Category: cannabinoid 1 receptor (cb1)]]
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| [[Category: helix while bound]]
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| [[Category: transferred no]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:58 2008''
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