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==Crystal structure of the nucleocapsid protein from Bunyamwera virus bound to RNA==
==Crystal structure of the nucleocapsid protein from Bunyamwera virus bound to RNA==
<StructureSection load='3zla' size='340' side='right' caption='[[3zla]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='3zla' size='340' side='right'caption='[[3zla]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3zla]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Bunv Bunv] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZLA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZLA FirstGlance]. <br>
<table><tr><td colspan='2'>[[3zla]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bunyamwera_virus Bunyamwera virus] and [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZLA FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zl9|3zl9]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zla OCA], [http://pdbe.org/3zla PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zla RCSB], [http://www.ebi.ac.uk/pdbsum/3zla PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zla ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zla OCA], [https://pdbe.org/3zla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zla RCSB], [https://www.ebi.ac.uk/pdbsum/3zla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zla ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/NCAP_BUNYW NCAP_BUNYW]
All orthobunyaviruses possess three genome segments of single-stranded negative sense RNA that are encapsidated with the virus-encoded nucleocapsid (N) protein to form a ribonucleoprotein (RNP) complex, which is uncharacterized at high resolution. We report the crystal structure of both the Bunyamwera virus (BUNV) N-RNA complex and the unbound Schmallenberg virus (SBV) N protein, at resolutions of 3.20 and 2.75 A, respectively. Both N proteins crystallized as ring-like tetramers and exhibit a high degree of structural similarity despite classification into different orthobunyavirus serogroups. The structures represent a new RNA-binding protein fold. BUNV N possesses a positively charged groove into which RNA is deeply sequestered, with the bases facing away from the solvent. This location is highly inaccessible, implying that RNA polymerization and other critical base pairing events in the virus life cycle require RNP disassembly. Mutational analysis of N protein supports a correlation between structure and function. Comparison between these crystal structures and electron microscopy images of both soluble tetramers and authentic RNPs suggests the N protein does not bind RNA as a repeating monomer; thus, it represents a newly described architecture for bunyavirus RNP assembly, with implications for many other segmented negative-strand RNA viruses.
 
Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization.,Ariza A, Tanner SJ, Walter CT, Dent KC, Shepherd DA, Wu W, Matthews SV, Hiscox JA, Green TJ, Luo M, Elliott RM, Fooks AR, Ashcroft AE, Stonehouse NJ, Ranson NA, Barr JN, Edwards TA Nucleic Acids Res. 2013 Apr 17. PMID:23595147<ref>PMID:23595147</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3zla" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Nucleoprotein|Nucleoprotein]]
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bunv]]
[[Category: Bunyamwera virus]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Ariza, A]]
[[Category: Ariza A]]
[[Category: Ashcroft, A E]]
[[Category: Ashcroft AE]]
[[Category: Barr, J N]]
[[Category: Barr JN]]
[[Category: Dent, K C]]
[[Category: Dent KC]]
[[Category: Edwards, T A]]
[[Category: Edwards TA]]
[[Category: Elliot, R M]]
[[Category: Elliot RM]]
[[Category: Green, T J]]
[[Category: Green TJ]]
[[Category: Hiscox, J A]]
[[Category: Hiscox JA]]
[[Category: Luo, M]]
[[Category: Luo M]]
[[Category: Matthews, S V]]
[[Category: Matthews SV]]
[[Category: Ranson, N A]]
[[Category: Ranson NA]]
[[Category: Shepherd, D A]]
[[Category: Shepherd DA]]
[[Category: Stonehouse, N J]]
[[Category: Stonehouse NJ]]
[[Category: Tanner, S J]]
[[Category: Tanner SJ]]
[[Category: Walter, C T]]
[[Category: Walter CT]]
[[Category: Wu, W]]
[[Category: Wu W]]
[[Category: Viral protein-rna complex]]
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