1bwp: Difference between revisions

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New page: left|200px<br /> <applet load="1bwp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bwp, resolution 2.1Å" /> '''PROBING THE SUBSTRAT...
 
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[[Image:1bwp.gif|left|200px]]<br />
<applet load="1bwp" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1bwp, resolution 2.1&Aring;" />
'''PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULAR BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE'''<br />


==Overview==
==PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULAR BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE==
Platelet-activating factor acetylhydrolases (PAF-AHs) are unique PLA2s, which hydrolyze the sn-2 ester linkage in PAF-like phospholipids with a, marked preference for very short acyl chains, typically acetyl. The recent, solution of the crystal structure of the alpha(1) catalytic subunit of, isoform Ib of bovine brain intracellular PAF-AH at 1.7 A resolution paved, the way for a detailed examination of the molecular basis of substrate, specificity in this enzyme. The crystal structure suggests that the side, chains of Thr103, Leu48 and Leu194 are involved in substrate recognition., Three single site mutants (L48A, T103S and L194A) were overexpressed and, their structures were solved to 2.3 A resolution or better by X-ray, diffraction methods. Enzyme kinetics showed that, compared with ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10469831 (full description)]]
<StructureSection load='1bwp' size='340' side='right'caption='[[1bwp]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bwp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BWP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bwp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bwp OCA], [https://pdbe.org/1bwp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bwp RCSB], [https://www.ebi.ac.uk/pdbsum/1bwp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bwp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA1B3_BOVIN PA1B3_BOVIN] Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/1bwp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bwp ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1BWP is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.47 3.1.1.47]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BWP OCA]].
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Probing the substrate specificity of the intracellular brain platelet-activating factor acetylhydrolase., Ho YS, Sheffield PJ, Masuyama J, Arai H, Li J, Aoki J, Inoue K, Derewenda U, Derewenda ZS, Protein Eng. 1999 Aug;12(8):693-700. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10469831 10469831]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Aoki, J.]]
[[Category: Aoki J]]
[[Category: Arai, H.]]
[[Category: Arai H]]
[[Category: Derewenda, U.]]
[[Category: Derewenda U]]
[[Category: Derewenda, Z.]]
[[Category: Derewenda Z]]
[[Category: Ho, Y.S.]]
[[Category: Ho YS]]
[[Category: Inoue, K.]]
[[Category: Inoue K]]
[[Category: Li, J.]]
[[Category: Li J]]
[[Category: Masuyama, J.]]
[[Category: Masuyama J]]
[[Category: Sheffield, P.J.]]
[[Category: Sheffield PJ]]
[[Category: acetylhydrolase hydrolase]]
[[Category: lipid degradation]]
[[Category: platelet factor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 17:18:39 2007''

Latest revision as of 06:39, 7 February 2024

PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULAR BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE

1bwp, resolution 2.10Å

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