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==Crystal Structure of the cryptic polo box (CPB)of ZYG-1==
==Crystal Structure of the cryptic polo box (CPB)of ZYG-1==
<StructureSection load='4nkb' size='340' side='right' caption='[[4nkb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='4nkb' size='340' side='right'caption='[[4nkb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4nkb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NKB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NKB FirstGlance]. <br>
<table><tr><td colspan='2'>[[4nkb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NKB FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nk7|4nk7]], [[4g7n|4g7n]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nkb OCA], [https://pdbe.org/4nkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nkb RCSB], [https://www.ebi.ac.uk/pdbsum/4nkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nkb ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nkb OCA], [http://pdbe.org/4nkb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nkb RCSB], [http://www.ebi.ac.uk/pdbsum/4nkb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nkb ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/ZYG1_CAEEL ZYG1_CAEEL]] Protein kinase that plays a central role in centrosome duplication. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome.<ref>PMID:11371350</ref> <ref>PMID:15232593</ref> <ref>PMID:15665853</ref> 
[https://www.uniprot.org/uniprot/ZYG1_CAEEL ZYG1_CAEEL] Protein kinase that plays a central role in centrosome duplication. Paternal copy is required to regulate synthesis of daughter centrioles prior to fertilization. Maternal copy regulates centrosome duplication during later cell cycles. Functions upstream of sas-5 and sas-6, and is required for their localization to the centrosome.<ref>PMID:11371350</ref> <ref>PMID:15232593</ref> <ref>PMID:15665853</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plk4 family kinases control centriole assembly. Plk4s target mother centrioles through an interaction between their cryptic polo box (CPB) and acidic regions in the centriolar receptors SPD-2/Cep192 and/or Asterless/Cep152. Here, we report a crystal structure for the CPB of C. elegans ZYG-1, which forms a Z-shaped dimer containing an intermolecular beta sheet with an extended basic surface patch. Biochemical and in vivo analysis revealed that electrostatic interactions dock the ZYG-1 CPB basic patch onto the SPD-2-derived acidic region to promote ZYG-1 targeting and new centriole assembly. Analysis of a different crystal form of the Drosophila Plk4 (DmPlk4) CPB suggests that it also forms a Z-shaped dimer. Comparison of the ZYG-1 and DmPlk4 CPBs revealed structural changes in the ZYG-1 CPB that confer selectivity for binding SPD-2 over Asterless-derived acidic regions. Overall, our findings suggest a conserved mechanism for centriolar docking of Plk4 homologs that initiate daughter centriole assembly.
 
Structure of the C. elegans ZYG-1 Cryptic Polo Box Suggests a Conserved Mechanism for Centriolar Docking of Plk4 Kinases.,Shimanovskaya E, Viscardi V, Lesigang J, Lettman MM, Qiao R, Svergun DI, Round A, Oegema K, Dong G Structure. 2014 Aug 5;22(8):1090-104. doi: 10.1016/j.str.2014.05.009. Epub 2014, Jun 26. PMID:24980795<ref>PMID:24980795</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4nkb" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Caenorhabditis elegans]]
[[Category: Dong, G]]
[[Category: Large Structures]]
[[Category: Shimanovskaya, E]]
[[Category: Dong G]]
[[Category: Centriole biogenesis]]
[[Category: Shimanovskaya E]]
[[Category: Centrosome]]
[[Category: Cryptic polo box]]
[[Category: Transferase]]

Latest revision as of 12:33, 1 March 2024

Crystal Structure of the cryptic polo box (CPB)of ZYG-1

4nkb, resolution 2.30Å

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