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==Crystal Structure of H61A mutant TTHA0252 from Thermus thermophilus HB8==
==Crystal Structure of H61A mutant TTHA0252 from Thermus thermophilus HB8==
<StructureSection load='3a4y' size='340' side='right' caption='[[3a4y]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3a4y' size='340' side='right'caption='[[3a4y]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3a4y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2zdw 2zdw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3A4Y FirstGlance]. <br>
<table><tr><td colspan='2'>[[3a4y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2zdw 2zdw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A4Y FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2dkf|2dkf]], [[3idz|3idz]], [[3ie0|3ie0]], [[3ie1|3ie1]], [[3ie2|3ie2]], [[3iek|3iek]], [[3iel|3iel]], [[3iem|3iem]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA0252 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4y OCA], [https://pdbe.org/3a4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a4y RCSB], [https://www.ebi.ac.uk/pdbsum/3a4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4y ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3a4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a4y OCA], [http://pdbe.org/3a4y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3a4y RCSB], [http://www.ebi.ac.uk/pdbsum/3a4y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3a4y ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RNSE_THET8 RNSE_THET8]] Has endoribonuclease activity towards 23S and 16S rRNA (in vitro).  
[https://www.uniprot.org/uniprot/RNSE_THET8 RNSE_THET8] Has endoribonuclease activity towards 23S and 16S rRNA (in vitro).
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4y_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/3a4y_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
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==See Also==
==See Also==
*[[Ribonuclease|Ribonuclease]]
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
*[[Temp|Temp]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Thet8]]
[[Category: Large Structures]]
[[Category: Ishikawa, H]]
[[Category: Thermus thermophilus HB8]]
[[Category: Kuramitsu, S]]
[[Category: Ishikawa H]]
[[Category: Masui, R]]
[[Category: Kuramitsu S]]
[[Category: Nakagawa, N]]
[[Category: Masui R]]
[[Category: Structural genomic]]
[[Category: Nakagawa N]]
[[Category: Yokoyama, S]]
[[Category: Yokoyama S]]
[[Category: Endonuclease]]
[[Category: Hydrolase]]
[[Category: Metal-binding]]
[[Category: Metallo beta lactamase fold]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Nuclease]]
[[Category: Rna-binding]]
[[Category: Rrna processing]]
[[Category: Rsgi]]

Latest revision as of 14:10, 1 November 2023

Crystal Structure of H61A mutant TTHA0252 from Thermus thermophilus HB8

3a4y, resolution 2.50Å

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