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| [[Image:1ma8.gif|left|200px]]
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| {{Structure
| | ==A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine== |
| |PDB= 1ma8 |SIZE=350|CAPTION= <scene name='initialview01'>1ma8</scene>, resolution 1.3Å
| | <StructureSection load='1ma8' size='340' side='right'caption='[[1ma8]], [[Resolution|resolution]] 1.30Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=UMS:2'-METHYLSELENYL-2'-DEOXYURIDINE-5'-PHOSPHATE'>UMS</scene>
| | <table><tr><td colspan='2'>[[1ma8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MA8 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UMS:2-METHYLSELENYL-2-DEOXYURIDINE-5-PHOSPHATE'>UMS</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [https://pdbe.org/1ma8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB], [https://www.ebi.ac.uk/pdbsum/1ma8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ma8 ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ma8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ma8 OCA], [http://www.ebi.ac.uk/pdbsum/1ma8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ma8 RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine'''
| | [[Category: Carrasco N]] |
| | | [[Category: Du Q]] |
| | | [[Category: Egli M]] |
| ==Overview==
| | [[Category: Huang Z]] |
| Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.
| | [[Category: Teplova M]] |
| | | [[Category: Tereshko V]] |
| ==About this Structure==
| | [[Category: Wawrzak Z]] |
| 1MA8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MA8 OCA].
| | [[Category: Wilds CJ]] |
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| ==Reference==
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| Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12458077 12458077]
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| [[Category: Protein complex]] | |
| [[Category: Carrasco, N.]] | |
| [[Category: Du, Q.]] | |
| [[Category: Egli, M.]] | |
| [[Category: Huang, Z.]] | |
| [[Category: Teplova, M.]] | |
| [[Category: Tereshko, V.]] | |
| [[Category: Wawrzak, Z.]] | |
| [[Category: Wilds, C J.]] | |
| [[Category: 2'-methylseleno-uridine]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:13:26 2008''
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