1md0: Difference between revisions

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[[Image:1md0.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1==
|PDB= 1md0 |SIZE=350|CAPTION= <scene name='initialview01'>1md0</scene>, resolution 2.00&Aring;
<StructureSection load='1md0' size='340' side='right'caption='[[1md0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1md0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MD0 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE= ETS-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1md0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1md0 OCA], [https://pdbe.org/1md0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1md0 RCSB], [https://www.ebi.ac.uk/pdbsum/1md0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1md0 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[1k79|1K79]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1md0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1md0 OCA], [http://www.ebi.ac.uk/pdbsum/1md0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1md0 RCSB]</span>
[https://www.uniprot.org/uniprot/ETS1_MOUSE ETS1_MOUSE] Transcription factor.
}}
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1md0_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1md0 ConSurf].
<div style="clear:both"></div>


'''CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1'''
==See Also==
 
*[[Ets1|Ets1]]
 
__TOC__
==Overview==
</StructureSection>
The DNA-binding activity of the eukaryotic transcription factor Ets-1 (E26 avian erythroblastosis virus oncogene-E twenty-six) is negatively regulated by inhibitory regions that flank the ETS domain. Based on the results of solution studies, these N- and C-terminal inhibitory regions have been proposed to pack against the ETS domain and form an autoinhibitory module whose N terminus partially unfolds upon binding of Ets-1 to DNA. Mutations that disrupt autoinhibition of DNA binding also cause a structural change in the inhibitory region. We report here a crystallographic study of fragments of Ets-1 that provide structural details of the inhibitory module and the structural transition that accompanies DNA binding. The structures of free and DNA-bound Ets-1 fragments containing the ETS domain and the inhibitory regions confirm that the N-terminal inhibitory region contains two alpha-helices one of which unfolds upon Ets-1 binding to DNA. The observations from the crystal structure, coupled with mutagenesis experiments, allow us to propose a model for the inhibited form of Ets-1 and lend insight into the flexible interaction between Ets-1 and the acute myeloid leukemia 1 protein, AML1 (RUNX1).
[[Category: Large Structures]]
 
==About this Structure==
1MD0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MD0 OCA].
 
==Reference==
Structural analysis of the autoinhibition of Ets-1 and its role in protein partnerships., Garvie CW, Pufall MA, Graves BJ, Wolberger C, J Biol Chem. 2002 Nov 22;277(47):45529-36. Epub 2002 Sep 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12221090 12221090]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Garvie CW]]
[[Category: Garvie, C W.]]
[[Category: Graves BJ]]
[[Category: Graves, B J.]]
[[Category: Pufall MA]]
[[Category: Pufall, M A.]]
[[Category: Wolberger C]]
[[Category: Wolberger, C.]]
[[Category: autoinhibition]]
[[Category: transcription factor]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:14:37 2008''

Latest revision as of 07:43, 14 February 2024

CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1

1md0, resolution 2.00Å

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