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==Crystal structure of the Get5 carboxyl domain from S. cerevisiae==
==Crystal structure of the Get5 carboxyl domain from S. cerevisiae==
<StructureSection load='3vej' size='340' side='right' caption='[[3vej]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
<StructureSection load='3vej' size='340' side='right'caption='[[3vej]], [[Resolution|resolution]] 1.23&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3vej]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VEJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VEJ FirstGlance]. <br>
<table><tr><td colspan='2'>[[3vej]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VEJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VEJ FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.23&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2lnz|2lnz]], [[2lo0|2lo0]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GET5, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vej OCA], [https://pdbe.org/3vej PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vej RCSB], [https://www.ebi.ac.uk/pdbsum/3vej PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vej ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vej FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vej OCA], [http://pdbe.org/3vej PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vej RCSB], [http://www.ebi.ac.uk/pdbsum/3vej PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vej ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST]] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> 
[https://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tail-anchored trans-membrane proteins are targeted to membranes post-translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of tail-anchored proteins destined to the endoplasmic reticulum from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog, and comparison of these structures allows annotation of specific stabilizing interactions. Using solution x-ray scattering and the structures of all folded domains, we present a model of the full-length Get4/Get5 complex.
 
Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836<ref>PMID:22262836</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3vej" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Baker's yeast]]
[[Category: Large Structures]]
[[Category: Chartron, J W]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Clemons, W M]]
[[Category: Chartron JW]]
[[Category: Rao, M]]
[[Category: Clemons Jr WM]]
[[Category: Vandervelde, D G]]
[[Category: Rao M]]
[[Category: Alpha helical]]
[[Category: Vandervelde DG]]
[[Category: Dimerization]]
[[Category: Homodimerization]]
[[Category: Protein binding]]
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