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[[Image:1n2m.jpg|left|200px]]


{{Structure
==The S53A Proenzyme Structure of Methanococcus jannaschii.==
|PDB= 1n2m |SIZE=350|CAPTION= <scene name='initialview01'>1n2m</scene>, resolution 1.90&Aring;
<StructureSection load='1n2m' size='340' side='right'caption='[[1n2m]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>
<table><tr><td colspan='2'>[[1n2m]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N2M FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Arginine_decarboxylase Arginine decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.19 4.1.1.19] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
|GENE= MJ0316 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2m OCA], [https://pdbe.org/1n2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n2m RCSB], [https://www.ebi.ac.uk/pdbsum/1n2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n2m ProSAT]</span></td></tr>
|RELATEDENTRY=[[1mt1|1MT1]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2m OCA], [http://www.ebi.ac.uk/pdbsum/1n2m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n2m RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PDAD_METJA PDAD_METJA]
 
== Evolutionary Conservation ==
'''The S53A Proenzyme Structure of Methanococcus jannaschii.'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n2m_consurf.spt"</scriptWhenChecked>
The three-dimensional structure of pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii was determined at 1.4 A resolution. The pyruvoyl group of arginine decarboxylase is generated by an autocatalytic internal serinolysis reaction at Ser53 in the proenzyme resulting in two polypeptide chains. The structure of the nonprocessing S53A mutant was also determined. The active site of the processed enzyme unexpectedly contained the reaction product agmatine. The crystal structure confirms that arginine decarboxylase is a homotrimer. The protomer fold is a four-layer alphabetabetaalpha sandwich with topology similar to pyruvoyl-dependent histidine decarboxylase. Highly conserved residues Asn47, Ser52, Ser53, Ile54, and Glu109 are proposed to play roles in the self-processing reaction. Agmatine binding residues include the C terminus of the beta chain (Ser52) from one protomer and the Asp35 side chain and the Gly44 and Val46 carbonyl oxygen atoms from an adjacent protomer. Glu109 is proposed to play a catalytic role in the decarboxylation reaction.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1N2M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2M OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n2m ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii: crystal structures of the self-cleaved and S53A proenzyme forms., Tolbert WD, Graham DE, White RH, Ealick SE, Structure. 2003 Mar;11(3):285-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12623016 12623016]
</StructureSection>
[[Category: Arginine decarboxylase]]
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Single protein]]
[[Category: Ealick SE]]
[[Category: Ealick, S E.]]
[[Category: Graham DE]]
[[Category: Graham, D E.]]
[[Category: Tolbert WD]]
[[Category: Tolbert, W D.]]
[[Category: White RH]]
[[Category: White, R H.]]
[[Category: agmatine]]
[[Category: arginine decarboxylase]]
[[Category: pyruvate]]
[[Category: pyruvoyl group]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:33 2008''
Retrieved from "https://proteopedia.org/1n2m"