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New page: left|200px<br /> <applet load="1thj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1thj, resolution 2.8Å" /> '''CARBONIC ANHYDRASE F...
 
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[[Image:1thj.gif|left|200px]]<br />
<applet load="1thj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1thj, resolution 2.8&Aring;" />
'''CARBONIC ANHYDRASE FROM METHANOSARCINA'''<br />


==Overview==
==CARBONIC ANHYDRASE FROM METHANOSARCINA==
A carbonic anhydrase from the thermophilic archaeon Methanosarcina, thermophila that exhibits no significant sequence similarity to known, carbonic anhydrases has recently been characterized. Here we present the, structure of this enzyme, which adopts a left-handed parallel beta-helix, fold. This fold is of particular interest since it contains only, left-handed crossover connections between the parallel beta-strands, which, so far have been observed very infrequently. The active form of the enzyme, is a trimer with three zinc-containing active sites, each located at the, interface between two monomers. While the arrangement of active site, groups differs between this enzyme and the carbonic anhydrases from higher, vertebrates, there are structural similarities in the zinc coordination, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?8665839 (full description)]]
<StructureSection load='1thj' size='340' side='right'caption='[[1thj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1thj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila]. The January 2004 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Carbonic Anhydrase''  by Shuchismita Dutta and David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2004_1 10.2210/rcsb_pdb/mom_2004_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1THJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1thj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1thj OCA], [https://pdbe.org/1thj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1thj RCSB], [https://www.ebi.ac.uk/pdbsum/1thj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1thj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAH_METTT CAH_METTT] Reversible hydration of carbon dioxide. Important for growth on acetate (PubMed:8041719). As a probably extracellular enzyme, it may support a H(+)/CH(3)COO(-) symport mechanism and/or conversion of CO(2) to HCO(3)(-), removing excess CO(2) produced by growth on acetate (Probable).<ref>PMID:8041719</ref> <ref>PMID:10924115</ref> <ref>PMID:8041719</ref> <ref>PMID:8665839</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/th/1thj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1thj ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1THJ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Methanosarcina_thermophila Methanosarcina thermophila]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. The following page contains interesting information on the relation of 1THJ with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb49_1.html Carbonic Anhydrase]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.1 4.2.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1THJ OCA]].
*[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]]
 
== References ==
==Reference==
<references/>
A left-hand beta-helix revealed by the crystal structure of a carbonic anhydrase from the archaeon Methanosarcina thermophila., Kisker C, Schindelin H, Alber BE, Ferry JG, Rees DC, EMBO J. 1996 May 15;15(10):2323-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8665839 8665839]
__TOC__
</StructureSection>
[[Category: Carbonic Anhydrase]]
[[Category: Carbonic Anhydrase]]
[[Category: Large Structures]]
[[Category: Methanosarcina thermophila]]
[[Category: Methanosarcina thermophila]]
[[Category: Single protein]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Kisker, C.]]
[[Category: Kisker C]]
[[Category: Rees, D.C.]]
[[Category: Rees DC]]
[[Category: Schindelin, H.]]
[[Category: Schindelin H]]
[[Category: ZN]]
[[Category: carbonic anhydrase]]
[[Category: lyase (oxo-acid)]]
 
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