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[[Image:1n5s.gif|left|200px]]


{{Structure
==Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Acetyl Dithranol==
|PDB= 1n5s |SIZE=350|CAPTION= <scene name='initialview01'>1n5s</scene>, resolution 1.70&Aring;
<StructureSection load='1n5s' size='340' side='right'caption='[[1n5s]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=1PG:2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL'>1PG</scene>, <scene name='pdbligand=ADL:(1,8-DIHYDROXY-9-OXO-9,10-DIHYDRO-ANTHRACEN-2-YL)-ACETIC+ACID'>ADL</scene>
<table><tr><td colspan='2'>[[1n5s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N5S FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
|GENE= ActVA-Orf6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PG:2-(2-{2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXY}-ETHOXY)-ETHANOL'>1PG</scene>, <scene name='pdbligand=ADL:(1,8-DIHYDROXY-9-OXO-9,10-DIHYDRO-ANTHRACEN-2-YL)-ACETIC+ACID'>ADL</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5s OCA], [https://pdbe.org/1n5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n5s RCSB], [https://www.ebi.ac.uk/pdbsum/1n5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n5s ProSAT]</span></td></tr>
|RELATEDENTRY=[[1lq9|1LQ9]], [[1n5q|1N5Q]], [[1n5t|1N5T]], [[1n5v|1N5V]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5s OCA], [http://www.ebi.ac.uk/pdbsum/1n5s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n5s RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q53908_STRCH Q53908_STRCH]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n5/1n5s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n5s ConSurf].
<div style="clear:both"></div>


'''Crystal structure of a Monooxygenase from the gene ActVA-Orf6 of Streptomyces coelicolor in complex with the ligand Acetyl Dithranol'''
==See Also==
 
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
ActVA-Orf6 monooxygenase from Streptomyces coelicolor that catalyses the oxidation of an aromatic intermediate of the actinorhodin biosynthetic pathway is a member of a class of small monooxygenases that carry out oxygenation without the assistance of any of the prosthetic groups, metal ions or cofactors normally associated with activation of molecular oxygen. The overall structure is a ferredoxin-like fold with a novel dimeric assembly, indicating that the widely represented ferredoxin fold may sustain yet another functionality. The resolution (1.3 A) of the enzyme structure and its complex with substrate and product analogues allows us to visualize the mechanism of binding and activation of the substrate for attack by molecular oxygen, and utilization of two gates for the reaction components including a proton gate and an O(2)/H(2)O gate with a putative protein channel. This is the first crystal structure of an enzyme involved in the tailoring of a type II aromatic polyketide and illustrates some of the enzyme-substrate recognition features that may apply to a range of other enzymes involved in modifying a polyketide core structure.
[[Category: Large Structures]]
 
[[Category: Federici L]]
==About this Structure==
[[Category: Kendrew SG]]
1N5S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N5S OCA].
[[Category: Malatesta F]]
 
[[Category: Marsh NG]]
==Reference==
[[Category: Miele AE]]
The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis., Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B, EMBO J. 2003 Jan 15;22(2):205-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12514126 12514126]
[[Category: Savino C]]
[[Category: Single protein]]
[[Category: Schimperna G]]
[[Category: Streptomyces coelicolor]]
[[Category: Sciara G]]
[[Category: Federici, L.]]
[[Category: Vallone B]]
[[Category: Kendrew, S G.]]
[[Category: Malatesta, F.]]
[[Category: Marsh, N G.]]
[[Category: Miele, A E.]]
[[Category: Savino, C.]]
[[Category: Schimperna, G.]]
[[Category: Sciara, G.]]
[[Category: Vallone, B.]]
[[Category: acetyl dithranol]]
[[Category: actinorhodin]]
[[Category: aromatic polyketide]]
[[Category: dihydrokalafungin]]
[[Category: monooxygenase]]
[[Category: streptomyces coelicolor]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:45 2008''
Retrieved from "https://proteopedia.org/1n5s"