Rhomboid protease: Difference between revisions

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== Function ==

'''Rhomboid protease''' (GlpG) is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins.

'''Rhomboid protease''' ('''GlpG''') is a transmembranal serine protease. The active site is buried in the lipid bilayer of cell membrane. It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>. Rhomboids regulate many cellular processes and are involved in many human diseases. The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer. Rhomboids are specific for a single transmembranal helix proteins.

*'''Rhomboid protease YqgP''' interacts with the membrane bound metlloprotease FisH and cleaves the high-affinity Mg transporter<ref>PMID:31930742</ref>.

== Structural highlights ==

The inhibitor isocoumarin binds to the GlpG catalytic residues Ser and His<ref>PMID:20890268</ref>.

The inhibitor <scene name='55/551211/Cv/5'>isocoumarin covalently binds to the GlpG catalytic residues Ser and His</scene> (in deepskyblue)<ref>PMID:20890268</ref>. <scene name='55/551211/Cv/6'>Whole active site</scene>. Water molecules shown as red spheres.

</StructureSection>

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{{#tree:id=OrganizedByTopic|openlevels=0|

*Rhomboid protease

*Rhomboid protease GlpG

**[[2ic8]], [[2irv]], [[2nrf]], [[2o7l]], [[3b45]], [[2xov]], [[4njn]], [[4njp]] – EcGlpG core region – ''Escherichia coli ''

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**[[2gqc]] – RP N terminal – NMR – ''Pseudomonas aeruginosa''

*Rhomboid protease complex with inhibitor

*Rhomboid protease GlpG complex with inhibitor

**[[2xow]], [[3zeb]] – EcGlpG core region + isocoumarin inhibitor

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**[[3ubb]] – EcGlpG core region + phosphonofluoridate inhibitor

**[[3zmh]], [[3zmi]], [[3zmj]], [[3zot]] – EcGlpG core region + β-lactam inhibitor

**[[5f5j]], [[5f5k]] – EcGlpG core region (mutant) + aldehyde inhibitor

**[[6pj5]], [[6pj7]], [[6pj8]], [[6pj9]], [[6pja]], [[6pjp]], [[6pjq]], [[6pjr]], [[6pju]] – EcGlpG core region + peptide aldehyde inhibitor

**[[5f5j]], [[5f5k]], [[6pj4]] – EcGlpG core region (mutant) + peptide aldehyde inhibitor

**[[4qo0]], [[4qo2]], [[5f5g]], [[5f5b]], [[5mtf]], [[5mt6]], [[5mt7]], [[5mt8]], [[6vj8]], [[6jv9]], [[6vjp]], [[6xro]], [[6xrp]] – EcGlpG core region + peptide inhibitor

**[[4qnz]] – EcGlpG core region (mutant) + peptide inhibitor

**[[4qo0]], [[4qo2]], [[5f5g]], [[5f5b]] – EcGlpG core region + peptide inhibitor

*Rhomboid protease YqgP

**[[6r0j]] – YqpG – ''Bacillus subtilis'' - NMR

*Rhomboid protease GluP

**[[7tj4]] – GluP + LytH – ''Staphylococcus aureus''

}}

== References ==

[[Category:Topic Page]]

Rhomboid protease: Difference between revisions

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-<StructureSection load='2xow' size='350' side='right' caption='Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry [[2xow]])' scene=''>
+<StructureSection load='' size='450' side='right' caption='Structure of rhomboid protease complex with inhibitor isocoumarin and nonyglucoside detergent molecules (PDB entry [[2xow]])' scene='55/551211/Cv/1'>
 == Function ==
-'''Rhomboid protease''' (GlpG) is a transmembranal serine protease.  The active site is buried in the lipid bilayer of cell membrane.  It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>.  Rhomboids regulate many cellular processes and are involved in many human diseases.  The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer.  Rhomboids are specific for a single transmembranal helix proteins.
+'''Rhomboid protease''' ('''GlpG''') is a transmembranal serine protease.  The active site is buried in the lipid bilayer of cell membrane.  It cleaves proteins within their transmembrane domain<ref>PMID:18605900</ref>.  Rhomboids regulate many cellular processes and are involved in many human diseases.  The catalytic site of rhomboids contains a hydrophilic pocket protected from the lipid bilayer.  Rhomboids are specific for a single transmembranal helix proteins.
+*'''Rhomboid protease YqgP''' interacts with the membrane bound metlloprotease FisH and cleaves the high-affinity Mg transporter<ref>PMID:31930742</ref>.
 == Structural highlights ==
-The inhibitor isocoumarin binds to the GlpG catalytic residues Ser and His<ref>PMID:20890268</ref>.
+The inhibitor <scene name='55/551211/Cv/5'>isocoumarin covalently binds to the GlpG catalytic residues Ser and His</scene> (in deepskyblue)<ref>PMID:20890268</ref>. <scene name='55/551211/Cv/6'>Whole active site</scene>. Water molecules shown as red spheres.
 </StructureSection>
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 {{#tree:id=OrganizedByTopic|openlevels=0|
-*Rhomboid protease
+*Rhomboid protease GlpG
 **[[2ic8]], [[2irv]], [[2nrf]], [[2o7l]], [[3b45]], [[2xov]], [[4njn]], [[4njp]] – EcGlpG core region – ''Escherichia coli ''  <BR />
@@ Line 22: / Line 23: @@
 **[[2gqc]] – RP N terminal – NMR – ''Pseudomonas aeruginosa''<br />
-*Rhomboid protease complex with inhibitor
+*Rhomboid protease GlpG complex with inhibitor
 **[[2xow]], [[3zeb]] – EcGlpG core region + isocoumarin inhibitor  <BR />
@@ Line 28: / Line 29: @@
 **[[3ubb]] – EcGlpG core region + phosphonofluoridate inhibitor  <BR />
 **[[3zmh]], [[3zmi]], [[3zmj]], [[3zot]] – EcGlpG core region + β-lactam inhibitor  <BR />
-**[[5f5j]], [[5f5k]] – EcGlpG core region (mutant) + aldehyde inhibitor  <BR />
+**[[6pj5]], [[6pj7]], [[6pj8]], [[6pj9]], [[6pja]], [[6pjp]], [[6pjq]], [[6pjr]], [[6pju]] – EcGlpG core region + peptide aldehyde inhibitor  <BR />
+**[[5f5j]], [[5f5k]], [[6pj4]] – EcGlpG core region (mutant) + peptide aldehyde inhibitor  <BR />
+**[[4qo0]], [[4qo2]], [[5f5g]], [[5f5b]], [[5mtf]], [[5mt6]], [[5mt7]], [[5mt8]], [[6vj8]], [[6jv9]], [[6vjp]], [[6xro]], [[6xrp]] – EcGlpG core region + peptide inhibitor  <BR />
 **[[4qnz]] – EcGlpG core region (mutant) + peptide inhibitor  <BR />
-**[[4qo0]], [[4qo2]], [[5f5g]], [[5f5b]] – EcGlpG core region + peptide inhibitor  <BR />
+*Rhomboid protease YqgP
+**[[6r0j]] – YqpG – ''Bacillus subtilis'' - NMR   <BR />
+*Rhomboid protease GluP
+**[[7tj4]] – GluP + LytH – ''Staphylococcus aureus''   <BR />
 }}
 == References ==
 <references/>
 [[Category:Topic Page]]