1n96: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
 
(10 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1n96.gif|left|200px]]


{{Structure
==DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)==
|PDB= 1n96 |SIZE=350|CAPTION= <scene name='initialview01'>1n96</scene>
<StructureSection load='1n96' size='340' side='right'caption='[[1n96]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
<table><tr><td colspan='2'>[[1n96]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N96 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n96 OCA], [https://pdbe.org/1n96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n96 RCSB], [https://www.ebi.ac.uk/pdbsum/1n96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n96 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[1eu2|1EU2]], [[1eu6|1EU6]]
<div style="background-color:#fffaf0;">
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n96 OCA], [http://www.ebi.ac.uk/pdbsum/1n96 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n96 RCSB]</span>
== Publication Abstract from PubMed ==
}}
 
'''DIMERIC SOLUTION STRUCTURE OF THE CYCLIC OCTAMER CD(CGCTCATT)'''
 
 
==Overview==
The solution structure of a cyclic oligonucleotide d&lt;pCGCTCATT&gt; has been determined by two-dimensional NMR spectroscopy and restrained molecular dynamics. Under the appropriate experimental conditions, this molecule self-associates, forming a symmetric dimer stabilized by four intermolecular Watson-Crick base pairs. The resulting four-stranded structure consists of two G:C:A:T tetrads, formed by facing the minor groove side of the Watson-Crick base-pairs. Most probably, the association of the base-pairs is stabilized by coordinating a Na(+) cation. This is the first time that this novel G:C:A:T tetrad has been found in an oligonucleotide structure. This observation increases considerably the number of sequences that may adopt a four-stranded architecture. Overall, the three-dimensional structure is similar to those observed previously in other quadruplexes formed by minor groove alignment of Watson-Crick base pairs. This resemblance strongly suggests that we may be observing a general motif for DNA-DNA recognition.
The solution structure of a cyclic oligonucleotide d&lt;pCGCTCATT&gt; has been determined by two-dimensional NMR spectroscopy and restrained molecular dynamics. Under the appropriate experimental conditions, this molecule self-associates, forming a symmetric dimer stabilized by four intermolecular Watson-Crick base pairs. The resulting four-stranded structure consists of two G:C:A:T tetrads, formed by facing the minor groove side of the Watson-Crick base-pairs. Most probably, the association of the base-pairs is stabilized by coordinating a Na(+) cation. This is the first time that this novel G:C:A:T tetrad has been found in an oligonucleotide structure. This observation increases considerably the number of sequences that may adopt a four-stranded architecture. Overall, the three-dimensional structure is similar to those observed previously in other quadruplexes formed by minor groove alignment of Watson-Crick base pairs. This resemblance strongly suggests that we may be observing a general motif for DNA-DNA recognition.


==About this Structure==
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad.,Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:12733903<ref>PMID:12733903</ref>
1N96 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N96 OCA].
 
==Reference==
Four-stranded DNA structure stabilized by a novel G:C:A:T tetrad., Escaja N, Gelpi JL, Orozco M, Rico M, Pedroso E, Gonzalez C, J Am Chem Soc. 2003 May 14;125(19):5654-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12733903 12733903]
[[Category: Protein complex]]
[[Category: Escaja, N.]]
[[Category: Gelpi, J L.]]
[[Category: Gonzalez, C.]]
[[Category: Orozco, M.]]
[[Category: Pedroso, E.]]
[[Category: Rico, M.]]
[[Category: bi-loop]]
[[Category: four-stranded dna]]
[[Category: quadruplex]]
[[Category: unusual dna]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:27:09 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1n96" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Escaja N]]
[[Category: Gelpi JL]]
[[Category: Gonzalez C]]
[[Category: Orozco M]]
[[Category: Pedroso E]]
[[Category: Rico M]]
Retrieved from "https://proteopedia.org/1n96"