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==Crystal Structure of Ribose 5-Phosphate Isomerase from the Probiotic Bacterium Lactobacillus salivarius UCC118==
==Crystal Structure of Ribose 5-Phosphate Isomerase from the Probiotic Bacterium Lactobacillus salivarius UCC118==
<StructureSection load='4gmk' size='340' side='right' caption='[[4gmk]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
<StructureSection load='4gmk' size='340' side='right'caption='[[4gmk]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4gmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacs1 Lacs1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GMK FirstGlance]. <br>
<table><tr><td colspan='2'>[[4gmk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ligilactobacillus_salivarius_UCC118 Ligilactobacillus salivarius UCC118]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GMK FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=362948 LACS1])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribose-5-phosphate_isomerase Ribose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.6 5.3.1.6] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmk OCA], [https://pdbe.org/4gmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gmk RCSB], [https://www.ebi.ac.uk/pdbsum/4gmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gmk ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmk OCA], [http://pdbe.org/4gmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gmk RCSB], [http://www.ebi.ac.uk/pdbsum/4gmk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gmk ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/Q1WR87_LACS1 Q1WR87_LACS1]] Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.[HAMAP-Rule:MF_00170][SAAS:SAAS00020827]
[https://www.uniprot.org/uniprot/Q1WR87_LIGS1 Q1WR87_LIGS1] Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.[HAMAP-Rule:MF_00170]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC188 has been determined at 1.72 A resolution. The structure was solved by molecular replacement, which identified the functional homodimer in the asymmetric unit. Despite only showing 57% sequence identity to its closest homologue, the structure adopted the typical alpha and beta D-ribose 5-phosphate isomerase fold. Comparison to other related structures revealed high homology in the active site, allowing a model of the substrate-bound protein to be proposed. The determination of the structure was expedited by the use of in situ crystallization-plate screening on beamline I04-1 at Diamond Light Source to identify well diffracting protein crystals prior to routine cryocrystallography.
 
Structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC118.,Lobley CM, Aller P, Douangamath A, Reddivari Y, Bumann M, Bird LE, Nettleship JE, Brandao-Neto J, Owens RJ, O'Toole PW, Walsh MA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1427-33., doi: 10.1107/S174430911204273X. Epub 2012 Nov 14. PMID:23192019<ref>PMID:23192019</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4gmk" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Ribose-5-phosphate isomerase|Ribose-5-phosphate isomerase]]
*[[Ribose-5-phosphate isomerase 3D structures|Ribose-5-phosphate isomerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Lacs1]]
[[Category: Large Structures]]
[[Category: Ribose-5-phosphate isomerase]]
[[Category: Ligilactobacillus salivarius UCC118]]
[[Category: Aller, P]]
[[Category: Aller P]]
[[Category: Bird, L E]]
[[Category: Bird LE]]
[[Category: Brandao-Neto, J]]
[[Category: Brandao-Neto J]]
[[Category: Bumann, M]]
[[Category: Bumann M]]
[[Category: Douangamath, A]]
[[Category: Douangamath A]]
[[Category: Lobley, C M.C]]
[[Category: Lobley CMC]]
[[Category: Owens, R J]]
[[Category: O'Toole PW]]
[[Category: Reddivari, Y]]
[[Category: Owens RJ]]
[[Category: Toole, P W.O]]
[[Category: Reddivari Y]]
[[Category: Walsh, M A]]
[[Category: Walsh MA]]
[[Category: D-ribose-5-phosphate isomerase family]]
[[Category: Isomerase]]
[[Category: Ribose 5-phosphate isomerisation]]
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