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==Crystal structure of epoxide hydrolase MonBI==
==Crystal structure of epoxide hydrolase MonBI==
<StructureSection load='3wmd' size='340' side='right' caption='[[3wmd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3wmd' size='340' side='right'caption='[[3wmd]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3wmd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1619 As 4.1619]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WMD FirstGlance]. <br>
<table><tr><td colspan='2'>[[3wmd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_virginiae Streptomyces virginiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WMD FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">monBI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1900 AS 4.1619])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.999&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmd OCA], [http://pdbe.org/3wmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wmd RCSB], [http://www.ebi.ac.uk/pdbsum/3wmd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmd ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wmd OCA], [https://pdbe.org/3wmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wmd RCSB], [https://www.ebi.ac.uk/pdbsum/3wmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wmd ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q846W7_STRVG Q846W7_STRVG]
Multistep catalysis of epoxide hydrolase/cyclase in the epoxide opening cascade is an intriguing issue in polyether biosynthesis. A pair of structurally homologous epoxide hydrolases was found in gene clusters of ionophore polyethers. In the epoxide opening reactions with MonBI and MonBII involved in monensin biosynthesis, we found that MonBII and catalytically inactive MonBI mutant catalyzed two-step reactions of bisepoxide substrate analog to afford bicyclic product although MonBII alone catalyzed only the first cyclization. The X-ray crystal structure of MonBI dimers suggested the importance of the KSD motif in MonBI/MonBI interaction, which was further supported by gel filtration chromatography of wild-type MonBI and mutant MonBI. The involvement of the KSD motif in heterodimer formation was confirmed by in vitro assay. Direct evidence of MonBI/MonBII interaction was obtained by native mass spectrometry. Its dissociation constant was determined as 2.21x10-5 M by surface plasmon resonance. Our results suggested the involvement of an allosteric regulation mechanism by MonBI/MonBII interaction in monensin skeletal construction.
 
Allosteric regulation of epoxide opening cascades by a pair of epoxide hydrolases in monensin biosynthesis.,Minami A, Ose T, Sato K, Oikawa A, Kuroki K, Maenaka K, Oguri H, Oikawa H ACS Chem Biol. 2013 Dec 9. PMID:24320215<ref>PMID:24320215</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3wmd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: As 4 1619]]
[[Category: Large Structures]]
[[Category: Kuroki, K]]
[[Category: Streptomyces virginiae]]
[[Category: Maenaka, K]]
[[Category: Kuroki K]]
[[Category: Minami, A]]
[[Category: Maenaka K]]
[[Category: Oguri, H]]
[[Category: Minami A]]
[[Category: Oikawa, A]]
[[Category: Oguri H]]
[[Category: Oikawa, H]]
[[Category: Oikawa A]]
[[Category: Ose, T]]
[[Category: Oikawa H]]
[[Category: Sato, K]]
[[Category: Ose T]]
[[Category: Epoxide-opening cyclic ether formation]]
[[Category: Sato K]]
[[Category: Isomerase]]
[[Category: Ntf2-like]]

Latest revision as of 08:45, 20 March 2024

Crystal structure of epoxide hydrolase MonBI

3wmd, resolution 2.00Å

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