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| [[Image:1obq.gif|left|200px]]
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| {{Structure
| | ==Apocrustacyanin C1 crystals grown in space and earth using vapour diffusion geometry== |
| |PDB= 1obq |SIZE=350|CAPTION= <scene name='initialview01'>1obq</scene>, resolution 1.85Å
| | <StructureSection load='1obq' size='340' side='right'caption='[[1obq]], [[Resolution|resolution]] 1.85Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1obq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OBQ FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1obq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obq OCA], [https://pdbe.org/1obq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1obq RCSB], [https://www.ebi.ac.uk/pdbsum/1obq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1obq ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1obq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1obq OCA], [http://www.ebi.ac.uk/pdbsum/1obq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1obq RCSB]</span>
| | [https://www.uniprot.org/uniprot/CRC1_HOMGA CRC1_HOMGA] Binds the carotenoid astaxanthin (AXT) which provides the blue coloration to the carapace of the lobster. |
| }}
| | == Evolutionary Conservation == |
| | | [[Image:Consurf_key_small.gif|200px|right]] |
| '''APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY'''
| | Check<jmol> |
| | | <jmolCheckbox> |
| | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/1obq_consurf.spt"</scriptWhenChecked> |
| ==Overview== | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| Models of apocrustacyanin C(1) were refined against X-ray data recorded on Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a space-grown and an earth-grown crystal, respectively, both using vapour-diffusion crystal-growth geometry. The space crystals were grown in the APCF on the NASA Space Shuttle. The microgravity crystal growth showed a cyclic nature attributed to Marangoni convection, thus reducing the benefits of the microgravity environment, as reported previously [Chayen et al. (1996), Q. Rev. Biophys. 29, 227-278]. A subsequent mosaicity evaluation, also reported previously, showed only a partial improvement in the space-grown crystals over the earth-grown crystals [Snell et al. (1997), Acta Cryst. D53, 231-239], contrary to the case for lysozyme crystals grown in space with liquid-liquid diffusion, i.e. without any major motion during growth [Snell et al. (1995), Acta Cryst. D52, 1099-1102]. In this paper, apocrustacyanin C(1) electron-density maps from the two refined models are now compared. It is concluded that the electron-density maps of the protein and the bound waters are found to be better overall for the structures of apocrustacyanin C(1) studied from the space-grown crystal compared with those from the earth-grown crystal, even though both crystals were grown using vapour-diffusion crystal-growth geometry. The improved residues are on the surface of the protein, with two involved in or nearby crystal lattice-forming interactions, thus linking an improved crystal-growth mechanism to the molecular level. The structural comparison procedures developed should themselves be valuable for evaluating crystal-growth procedures in the future.
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | | </jmolCheckbox> |
| ==About this Structure== | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1obq ConSurf]. |
| 1OBQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homarus_gammarus Homarus gammarus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OBQ OCA].
| | <div style="clear:both"></div> |
| | | __TOC__ |
| ==Reference==
| | </StructureSection> |
| Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12832753 12832753]
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| [[Category: Homarus gammarus]] | | [[Category: Homarus gammarus]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Boggon, T J.]] | | [[Category: Boggon TJ]] |
| [[Category: Chayen, N E.]] | | [[Category: Chayen NE]] |
| [[Category: Habash, J.]] | | [[Category: Habash J]] |
| [[Category: Helliwell, J R.]] | | [[Category: Helliwell JR]] |
| [[Category: Raftery, J.]] | | [[Category: Raftery J]] |
| [[Category: Zagalsky, P F.]] | | [[Category: Zagalsky PF]] |
| [[Category: antiparallel beta-strand]]
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| [[Category: lipocalin]]
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| [[Category: pigment]]
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| [[Category: transport protein]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:42:53 2008''
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