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| [[Image:1ofx.jpg|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION== |
| |PDB= 1ofx |SIZE=350|CAPTION= <scene name='initialview01'>1ofx</scene>, resolution 2.000Å
| | <StructureSection load='1ofx' size='340' side='right'caption='[[1ofx]], [[Resolution|resolution]] 2.00Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene>
| | <table><tr><td colspan='2'>[[1ofx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OFX FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ofx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofx OCA], [https://pdbe.org/1ofx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ofx RCSB], [https://www.ebi.ac.uk/pdbsum/1ofx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofx ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ofx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofx OCA], [http://www.ebi.ac.uk/pdbsum/1ofx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ofx RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''
| | [[Category: Egli M]] |
| | | [[Category: Rich A]] |
| | | [[Category: Usman N]] |
| ==Overview==
| | [[Category: Zhang S]] |
| In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.
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| ==About this Structure==
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| 1OFX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA].
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| ==Reference==
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| Crystal structure of an Okazaki fragment at 2-A resolution., Egli M, Usman N, Zhang SG, Rich A, Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):534-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1370582 1370582]
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| [[Category: Protein complex]] | |
| [[Category: Egli, M.]] | |
| [[Category: Rich, A.]] | |
| [[Category: Usman, N.]] | |
| [[Category: Zhang, S.]] | |
| [[Category: a-dna/rna]]
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| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:44:44 2008''
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