5gu4: Difference between revisions
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New page: '''Unreleased structure''' The entry 5gu4 is ON HOLD Authors: Description: Category: Unreleased Structures |
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==rRNA N-glycosylase RTA== | |||
<StructureSection load='5gu4' size='340' side='right'caption='[[5gu4]], [[Resolution|resolution]] 1.55Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5gu4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GU4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gu4 OCA], [https://pdbe.org/5gu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gu4 RCSB], [https://www.ebi.ac.uk/pdbsum/5gu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gu4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RLA2_HUMAN RLA2_HUMAN] Plays an important role in the elongation step of protein synthesis.[HAMAP-Rule:MF_01478] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ricin is a type 2 ribosome-inactivating protein (RIP), containing a catalytic A chain and a lectin-like B chain. It inhibits protein synthesis by depurinating the N-glycosidic bond at alpha-sarcin/ricin loop (SRL) of the 28S rRNA, which thereby prevents the binding of elongation factors to the GTPase activation center of the ribosome. Here, we present the 1.6 A crystal structure of Ricin A chain (RTA) complexed to the C-terminal peptide of the ribosomal stalk protein P2, which plays a crucial role in specific recognition of elongation factors and recruitment of eukaryote-specific RIPs to the ribosomes. Our structure reveals that the C-terminal GFGLFD motif of P2 peptide is inserted into a hydrophobic pocket of RTA, while the interaction assays demonstrate the structurally untraced SDDDM motif of P2 peptide contributes to the interaction with RTA. This interaction mode of RTA and P protein is in contrast to that with trichosanthin (TCS), Shiga-toxin (Stx) and the active form of maize RIP (MOD), implying the flexibility of the P2 peptide-RIP interaction, for the latter to gain access to ribosome. | |||
Crystal Structure of Ribosome-Inactivating Protein Ricin A Chain in Complex with the C-Terminal Peptide of the Ribosomal Stalk Protein P2.,Shi WW, Tang YS, Sze SY, Zhu ZN, Wong KB, Shaw PC Toxins (Basel). 2016 Oct 13;8(10). pii: E296. PMID:27754366<ref>PMID:27754366</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5gu4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ricin 3D structures|Ricin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Ricinus communis]] | |||
[[Category: Shaw PC]] | |||
[[Category: Shi WW]] | |||
[[Category: Sze SY]] | |||
[[Category: Tang YS]] | |||
[[Category: Wong KB]] | |||
[[Category: Zhu ZN]] | |||
