5t20: Difference between revisions
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New page: '''Unreleased structure''' The entry 5t20 is ON HOLD until Paper Publication Authors: Pereira, P.R., Meagher, J.L., Stuckey, J.A. Description: Crystal Structure of Tarin Lectin bound t... |
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==Crystal Structure of Tarin Lectin bound to Trimannose== | |||
<StructureSection load='5t20' size='340' side='right'caption='[[5t20]], [[Resolution|resolution]] 1.91Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5t20]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Colocasia_esculenta Colocasia esculenta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T20 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.91Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=PRD_900118:6alpha-alpha-mannobiose'>PRD_900118</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t20 OCA], [https://pdbe.org/5t20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t20 RCSB], [https://www.ebi.ac.uk/pdbsum/5t20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t20 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LEC2_COLES LEC2_COLES] Mannose-specific lectin (PubMed:27558840). Shows agglutinating activity towards erythrocytes from rabbit (By similarity).[UniProtKB:R9RL27]<ref>PMID:27558840</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tarin, the Colocasia esculenta lectin from the superfamily of alpha-d-mannose-specific plant bulb lectins, is a tetramer of 47 kDa composed of two heterodimers. Each heterodimer possesses homologous monomers of ~11.9 (A chain) and ~12.7 (B chain) kDa. The structures of apo and carbohydrate-bound tarin were solved to 1.7 A and 1.91 A, respectively. Each tarin monomer forms a canonical beta-prism II fold, common to all members of Galanthus nivalis agglutinin (GNA) family, which is partially stabilized by a disulfide bond and a conserved hydrophobic core. The heterodimer is formed through domain swapping involving the C-terminal beta-strand and the beta-sheet on face I of the prism. The tetramer is assembled through the dimerization of the B chains from heterodimers involving face II of each prism. The 1.91 A crystal structure of tarin bound to Manalpha(1,3)Manalpha(1,6)Man reveals an expanded carbohydrate-binding sequence (QxDxNxVxYx4/6WX) on face III of the beta-prism. Both monomers possess a similar fold, except for the length of the loop, which begins after the conserved tyrosine and creates the binding pocket for the alpha(1,6)-terminal mannose. This loop differs in size and amino-acid composition from 10 other beta-prism II domain proteins, and may confer carbohydrate-binding specificity among members of the GNA-related lectin family. | |||
High-resolution crystal structures of Colocasia esculenta tarin lectin.,Pereira PR, Meagher JL, Winter HC, Goldstein IJ, Paschoalin VM, Silva JT, Stuckey JA Glycobiology. 2016 Aug 24. PMID:27558840<ref>PMID:27558840</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Meagher | <div class="pdbe-citations 5t20" style="background-color:#fffaf0;"></div> | ||
[[Category: Pereira | == References == | ||
[[Category: Stuckey | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Colocasia esculenta]] | |||
[[Category: Large Structures]] | |||
[[Category: Meagher JL]] | |||
[[Category: Pereira PR]] | |||
[[Category: Stuckey JA]] | |||
Latest revision as of 12:53, 4 October 2023
Crystal Structure of Tarin Lectin bound to Trimannose
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