5t5c: Difference between revisions
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New page: '''Unreleased structure''' The entry 5t5c is ON HOLD Authors: Michal R. Szymanski, Whitney Y. Yin Description: A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repai... |
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==A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme== | |||
<StructureSection load='5t5c' size='340' side='right'caption='[[5t5c]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5t5c]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/DNA_launch_vector_pDE-GFP2 DNA launch vector pDE-GFP2] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T5C FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.851Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t5c OCA], [https://pdbe.org/5t5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t5c RCSB], [https://www.ebi.ac.uk/pdbsum/5t5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t5c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/EXOG_HUMAN EXOG_HUMAN] Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity.<ref>PMID:18187503</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human EXOG (hEXOG) is a 5'-exonuclease that is crucial for mitochondrial DNA repair; the enzyme belongs to a nonspecific nuclease family that includes the apoptotic endonuclease EndoG. Here we report biochemical and structural studies of hEXOG, including structures in its apo form and in a complex with DNA at 1.81 and 1.85 A resolution, respectively. A Wing domain, absent in other betabetaalpha-Me members, suppresses endonuclease activity, but confers on hEXOG a strong 5'-dsDNA exonuclease activity that precisely excises a dinucleotide using an intrinsic 'tape-measure'. The symmetrical apo hEXOG homodimer becomes asymmetrical upon binding to DNA, providing a structural basis for how substrate DNA bound to one active site allosterically regulates the activity of the other. These properties of hEXOG suggest a pathway for mitochondrial BER that provides an optimal substrate for subsequent gap-filling synthesis by DNA polymerase gamma. | |||
A domain in human EXOG converts apoptotic endonuclease to DNA-repair exonuclease.,Szymanski MR, Yu W, Gmyrek AM, White MA, Molineux IJ, Lee JC, Yin YW Nat Commun. 2017 May 3;8:14959. doi: 10.1038/ncomms14959. PMID:28466855<ref>PMID:28466855</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5t5c" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: DNA launch vector pDE-GFP2]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Szymanski MR]] | |||
[[Category: Yin WY]] | |||
Latest revision as of 12:54, 4 October 2023
A Novel domain in human EXOG converts apoptotic endonuclease to DNA-repair enzyme
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