5hyc: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Structure based function annotation of a hypothetical protein MGG_01005 related to the development of rice blast fungus== | |||
<StructureSection load='5hyc' size='340' side='right'caption='[[5hyc]], [[Resolution|resolution]] 2.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5hyc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_oryzae_70-15 Pyricularia oryzae 70-15]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HYC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hyc OCA], [https://pdbe.org/5hyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hyc RCSB], [https://www.ebi.ac.uk/pdbsum/5hyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hyc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G4NCW2_MAGO7 G4NCW2_MAGO7] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Magnaporthe oryzae is a model fungal plant pathogen employed for studying plant-fungi interactions. Whole genome sequencing and bioinformatics analyses revealed that this fungal pathogen has more than 12,000 protein-coding genes with 65% of the genes remaining functionally un-annotated. Here, we determine the structure of the hypothetical protein, MGG_01005 and show that it is the Magnaporthe oryzae Dynein light chain Tctex-type 1 (dynlt1/3), demonstrated by its structural similarity to other orthologous dynlt1 proteins and its conserved interaction with the N-terminus of the Magnaporthe oryzae dynein intermediate chain, MoDyn1I2. In addition, we present the structure of the MGG_01005-MoDyn1I2 complex together with mutagenesis studies that reveals a di-histidine motif interaction with a glutamate residue in the dynein intermediate chain within a conserved molecular interface. These results demonstrate the utility of structure-based annotation and validate it as a viable approach for the molecular assignment of hypothetic proteins from phyto-pathogenic fungi. | |||
Structure based function-annotation of hypothetical protein MGG_01005 from Magnaporthe oryzae reveals it is the dynein light chain orthologue of dynlt1/3.,Li G, Huang J, Yang J, He D, Wang C, Qi X, Taylor IA, Liu J, Peng YL Sci Rep. 2018 Mar 2;8(1):3952. doi: 10.1038/s41598-018-21667-5. PMID:29500373<ref>PMID:29500373</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5hyc" style="background-color:#fffaf0;"></div> | ||
[[Category: Huang | |||
[[Category: Liu | ==See Also== | ||
[[Category: Peng | *[[Dynein 3D structures|Dynein 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pyricularia oryzae 70-15]] | |||
[[Category: Huang J]] | |||
[[Category: Li G]] | |||
[[Category: Liu J]] | |||
[[Category: Peng Y-l]] | |||
