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==Crystal structure of ph0300==
==Crystal structure of ph0300==
<StructureSection load='3vrh' size='340' side='right' caption='[[3vrh]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='3vrh' size='340' side='right'caption='[[3vrh]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3vrh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VRH FirstGlance]. <br>
<table><tr><td colspan='2'>[[3vrh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VRH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PH0300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=70601 Pyrococcus horikoshii])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCN:BICINE'>BCN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrh OCA], [http://pdbe.org/3vrh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vrh RCSB], [http://www.ebi.ac.uk/pdbsum/3vrh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vrh ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vrh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrh OCA], [https://pdbe.org/3vrh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vrh RCSB], [https://www.ebi.ac.uk/pdbsum/3vrh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vrh ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TTUA_PYRHO TTUA_PYRHO] Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T) (PubMed:28655838). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures (By similarity). Can use free sulfide as sulfur source in vitro, which may be also the sulfur source in vivo (PubMed:28655838).[UniProtKB:Q72LF3]<ref>PMID:28655838</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pyrococcus horikoshii]]
[[Category: Large Structures]]
[[Category: Goto-Ito, S]]
[[Category: Pyrococcus horikoshii OT3]]
[[Category: Ito, T]]
[[Category: Goto-Ito S]]
[[Category: Kuratani, M]]
[[Category: Ito T]]
[[Category: Nakagawa, H]]
[[Category: Kuratani M]]
[[Category: Sekine, S I]]
[[Category: Nakagawa H]]
[[Category: Yokoyama, S]]
[[Category: Sekine SI]]
[[Category: Atpase]]
[[Category: Yokoyama S]]
[[Category: Rna binding protein]]
[[Category: Thiolation]]
[[Category: Trna modification enzyme]]
Retrieved from "https://proteopedia.org/3vrh"