5h0r: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 5h0r is ~~ON HOLD~~

==RNA dependent RNA polymerase ,vp4,dsRNA==

== Structural highlights ==

<table><tr><td colspan='2'>[[5h0r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1], [https://en.wikipedia.org/wiki/Cypovirus Cypovirus] and [https://en.wikipedia.org/wiki/Dendrolimus_punctatus_cypovirus_1 Dendrolimus punctatus cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H0R FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h0r OCA], [https://pdbe.org/5h0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h0r RCSB], [https://www.ebi.ac.uk/pdbsum/5h0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h0r ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/A0A0S1LIW6_CPVBM A0A0S1LIW6_CPVBM]

== Publication Abstract from PubMed ==

Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5A) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-A diameter at 3.3-A resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-A resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-A resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed.

~~Authors:~~ Li, X., Zhou, N., Chen, W., Zhu, B., Wang, X., Xu, B., Wang, J., Liu, H., Cheng, L.

Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.,Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L J Mol Biol. 2017 Jan 6;429(1):79-87. doi: 10.1016/j.jmb.2016.11.025. Epub 2016, Dec 1. PMID:27914893<ref>PMID:27914893</ref>

~~Description~~: RNA ~~dependent~~ RNA polymerase ~~,vp4,dsRNA~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

[[Category: ~~Cheng, L~~]]

[[Category: ~~Liu, H~~]]

[[Category: Chen, W]]

==See Also==

[[Category: ~~Zhou, N~~]]

*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]

[[Category: ~~Wang,~~ X]]

*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]

[[Category: Wang, J]]

== References ==

[[Category: ~~Li,~~ X]]

[[Category: Xu, B]]

__TOC__

[[Category: Zhu, B]]

</SX>

[[Category: Bombyx mori cypovirus 1]]

[[Category: Cypovirus]]

[[Category: Dendrolimus punctatus cypovirus 1]]

[[Category: Large Structures]]

[[Category: Chen W]]

[[Category: Cheng L]]

[[Category: Li X]]

[[Category: Liu H]]

[[Category: Wang J]]

[[Category: Wang X]]

[[Category: Xu B]]

[[Category: Zhou N]]

[[Category: Zhu B]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5h0r is ON HOLD
+==RNA dependent RNA polymerase ,vp4,dsRNA==
+<SX load='5h0r' size='340' side='right' viewer='molstar' caption='[[5h0r]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5h0r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1], [https://en.wikipedia.org/wiki/Cypovirus Cypovirus] and [https://en.wikipedia.org/wiki/Dendrolimus_punctatus_cypovirus_1 Dendrolimus punctatus cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H0R FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h0r OCA], [https://pdbe.org/5h0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h0r RCSB], [https://www.ebi.ac.uk/pdbsum/5h0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h0r ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0S1LIW6_CPVBM A0A0S1LIW6_CPVBM]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Single-particle cryo-electron microscopy (cryo-EM) allows the high-resolution structural determination of biological assemblies in a near-native environment. However, all high-resolution (better than 3.5A) cryo-EM structures reported to date were obtained by using 300kV transmission electron microscopes (TEMs). We report here the structures of a cypovirus capsid of 750-A diameter at 3.3-A resolution and of RNA-dependent RNA polymerase (RdRp) complexes within the capsid at 3.9-A resolution using a 200-kV TEM. The newly resolved structure revealed conformational changes of two subdomains in the RdRp. These conformational changes, which were involved in RdRp's switch from non-transcribing to transcribing mode, suggest that the RdRp may facilitate the unwinding of genomic double-stranded RNA. The possibility of 3-A resolution structural determinations for biological assemblies of relatively small sizes using cryo-EM at 200kV was discussed.
-Authors: Li, X., Zhou, N., Chen, W., Zhu, B., Wang, X., Xu, B., Wang, J., Liu, H., Cheng, L.
+Near-Atomic Resolution Structure Determination of a Cypovirus Capsid and Polymerase Complex Using Cryo-EM at 200kV.,Li X, Zhou N, Chen W, Zhu B, Wang X, Xu B, Wang J, Liu H, Cheng L J Mol Biol. 2017 Jan 6;429(1):79-87. doi: 10.1016/j.jmb.2016.11.025. Epub 2016, Dec 1. PMID:27914893<ref>PMID:27914893</ref>
-Description: RNA dependent RNA polymerase ,vp4,dsRNA
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Cheng, L]]
+<div class="pdbe-citations 5h0r" style="background-color:#fffaf0;"></div>
-[[Category: Liu, H]]
-[[Category: Chen, W]]
+==See Also==
-[[Category: Zhou, N]]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-[[Category: Wang, X]]
+*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
-[[Category: Wang, J]]
+== References ==
-[[Category: Li, X]]
+<references/>
-[[Category: Xu, B]]
+__TOC__
-[[Category: Zhu, B]]
+</SX>
+[[Category: Bombyx mori cypovirus 1]]
+[[Category: Cypovirus]]
+[[Category: Dendrolimus punctatus cypovirus 1]]
+[[Category: Large Structures]]
+[[Category: Chen W]]
+[[Category: Cheng L]]
+[[Category: Li X]]
+[[Category: Liu H]]
+[[Category: Wang J]]
+[[Category: Wang X]]
+[[Category: Xu B]]
+[[Category: Zhou N]]
+[[Category: Zhu B]]