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[[Image:1plc.gif|left|200px]]


{{Structure
==ACCURACY AND PRECISION IN PROTEIN CRYSTAL STRUCTURE ANALYSIS: RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF POPLAR PLASTOCYANIN AT 1.33 ANGSTROMS RESOLUTION==
|PDB= 1plc |SIZE=350|CAPTION= <scene name='initialview01'>1plc</scene>, resolution 1.33&Aring;
<StructureSection load='1plc' size='340' side='right'caption='[[1plc]], [[Resolution|resolution]] 1.33&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>
<table><tr><td colspan='2'>[[1plc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1pcy 1pcy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PLC FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1plc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1plc OCA], [https://pdbe.org/1plc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1plc RCSB], [https://www.ebi.ac.uk/pdbsum/1plc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1plc ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1plc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1plc OCA], [http://www.ebi.ac.uk/pdbsum/1plc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1plc RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pl/1plc_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1plc ConSurf].
<div style="clear:both"></div>


'''ACCURACY AND PRECISION IN PROTEIN CRYSTAL STRUCTURE ANALYSIS: RESTRAINED LEAST-SQUARES REFINEMENT OF THE CRYSTAL STRUCTURE OF POPLAR PLASTOCYANIN AT 1.33 ANGSTROMS RESOLUTION'''
==See Also==
 
*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The structure of the electron-transfer protein, plastocyanin (99 amino acids, one Cu atom, 10,500 Da) from poplar leaves, has been refined at 1.33 A resolution to a residual R = 0.15. The space group is orthorhombic, P2(1)2(1)2(1), a = 29.60 (1), b = 46.86 (3), c = 57.60 (3) A. The 14,303 reflections used in the refinement were obtained from a data set recorded on a four-circle diffractometer with radiation from a sealed fine-focus tube, combined with a data set measured on oscillation films exposed at the DESY synchrotron. The final model comprises 1442 (738 non-H) protein atoms, one Cu atom and 110 solvent molecules. Nine residues are described as disordered. The root-mean-square deviation from ideal bond lengths is 0.016 A and the root-mean-square difference between the positions of the C alpha atoms in this refined model and in the structure previously refined at 1.6 A resolution is 0.11 A. The effects of manual model adjustment, resolution, choice of standard values for geometrical parameters, inclusion of H atoms and inclusion of anomalous-scattering corrections on the copper-site geometry have been explored. The final values of the Cu-ligand bond lengths are: Cu--N(His37) 1.91, Cu--S(Cys84) 2.07, Cu--N(His87) 2.06, Cu--S(Met92) 2.82 A.
[[Category: Large Structures]]
 
==About this Structure==
1PLC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. This structure supersedes the now removed PDB entry 1PCY. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PLC OCA].
 
==Reference==
Accuracy and precision in protein structure analysis: restrained least-squares refinement of the structure of poplar plastocyanin at 1.33 A resolution., Guss JM, Bartunik HD, Freeman HC, Acta Crystallogr B. 1992 Dec 1;48 ( Pt 6):790-811. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1492962 1492962]
[[Category: Populus nigra]]
[[Category: Populus nigra]]
[[Category: Single protein]]
[[Category: Freeman HC]]
[[Category: Freeman, H C.]]
[[Category: Guss JM]]
[[Category: Guss, J M.]]
[[Category: electron transport]]
 
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Retrieved from "https://proteopedia.org/1plc"