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[[Image:1pnn.gif|left|200px]]


{{Structure
==PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA==
|PDB= 1pnn |SIZE=350|CAPTION= <scene name='initialview01'>1pnn</scene>, resolution 2.5&Aring;
<StructureSection load='1pnn' size='340' side='right'caption='[[1pnn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-CYTOSINE'>CPN</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=IPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-5-IODOURACIL'>IPN</scene>, <scene name='pdbligand=TPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-THYMINE'>TPN</scene>
<table><tr><td colspan='2'>[[1pnn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PNN FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-CYTOSINE'>CPN</scene>, <scene name='pdbligand=IPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-5-IODOURACIL'>IPN</scene>, <scene name='pdbligand=TPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-THYMINE'>TPN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnn OCA], [https://pdbe.org/1pnn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pnn RCSB], [https://www.ebi.ac.uk/pdbsum/1pnn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pnn ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pnn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pnn OCA], [http://www.ebi.ac.uk/pdbsum/1pnn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pnn RCSB]</span>
<div style="background-color:#fffaf0;">
}}
== Publication Abstract from PubMed ==
 
'''PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA'''
 
 
==Overview==
The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.
The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.


==About this Structure==
A nucleic acid triple helix formed by a peptide nucleic acid-DNA complex.,Betts L, Josey JA, Veal JM, Jordan SR Science. 1995 Dec 15;270(5243):1838-41. PMID:8525381<ref>PMID:8525381</ref>
1PNN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNN OCA].
 
==Reference==
A nucleic acid triple helix formed by a peptide nucleic acid-DNA complex., Betts L, Josey JA, Veal JM, Jordan SR, Science. 1995 Dec 15;270(5243):1838-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8525381 8525381]
[[Category: Protein complex]]
[[Category: Betts, L.]]
[[Category: Veal, J M.]]
[[Category: hairpin pna:dna triplex]]
[[Category: triplex watson-crick hoogsteen]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:02:03 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1pnn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Betts L]]
[[Category: Veal JM]]

Latest revision as of 08:01, 15 November 2023

PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA

1pnn, resolution 2.50Å

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