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[[Image:1poy.jpg|left|200px]]


{{Structure
==SPERMIDINE/PUTRESCINE-BINDING PROTEIN COMPLEXED WITH SPERMIDINE (DIMER FORM)==
|PDB= 1poy |SIZE=350|CAPTION= <scene name='initialview01'>1poy</scene>, resolution 2.5&Aring;
<StructureSection load='1poy' size='340' side='right'caption='[[1poy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene>
<table><tr><td colspan='2'>[[1poy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1POY FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1poy OCA], [https://pdbe.org/1poy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1poy RCSB], [https://www.ebi.ac.uk/pdbsum/1poy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1poy ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1poy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1poy OCA], [http://www.ebi.ac.uk/pdbsum/1poy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1poy RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/POTD_ECOLI POTD_ECOLI] Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein.
 
== Evolutionary Conservation ==
'''SPERMIDINE/PUTRESCINE-BINDING PROTEIN COMPLEXED WITH SPERMIDINE (DIMER FORM)'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/po/1poy_consurf.spt"</scriptWhenChecked>
PotD protein is a periplasmic binding protein and the primary receptor of the polyamine transport system, which regulates the polyamine content in Escherichia coli. The crystal structure of PotD in complex with spermidine has been solved at 2.5-A resolution. The PotD protein consists of two domains with an alternating beta-alpha-beta topology. The polyamine binding site is in a central cleft lying in the interface between the domains. In the cleft, four acidic residues recognize the three positively charged nitrogen atoms of spermidine, while five aromatic side chains anchor the methylene backbone by van der Waals interactions. The overall fold of PotD is similar to that of other periplasmic binding proteins, and in particular to the maltodextrin-binding protein from E. coli, despite the fact that sequence identity is as low as 20%. The comparison of the PotD structure with the two maltodextrin-binding protein structures, determined in the presence and absence of the substrate, suggests that spermidine binding rearranges the relative orientation of the PotD domains to create a more compact structure.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1POY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POY OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1poy ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Crystal structure of PotD, the primary receptor of the polyamine transport system in Escherichia coli., Sugiyama S, Vassylyev DG, Matsushima M, Kashiwagi K, Igarashi K, Morikawa K, J Biol Chem. 1996 Apr 19;271(16):9519-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8621624 8621624]
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Matsushima, M.]]
[[Category: Matsushima M]]
[[Category: Morikawa, K.]]
[[Category: Morikawa K]]
[[Category: Sugiyama, S.]]
[[Category: Sugiyama S]]
[[Category: Vassylyev, D G.]]
[[Category: Vassylyev DG]]
[[Category: binding protein]]
[[Category: transport protein]]
 
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Retrieved from "https://proteopedia.org/1poy"