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| <StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' > | | <StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' > |
| == Function == | | == Function == |
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| *'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br /> | | *'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br /> |
| *'''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). <br /> | | *'''Tyr-recombinases''' include '''Cre''' which recombines loxP sites, FLP and lambda integrase (LamInt). <br /> |
| *'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br /> | | *'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br /> |
| *'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br /> | | *'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br /> |
| | *'''RadB recombinase''' is a paralogue of RadA assisting in RadA-mediated strand exchange <ref>PMID:28501701</ref>. <br /> |
| *'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>. | | *'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>. |
| </StructureSection> | | *'''Tre recombinase''' recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells <ref>PMID:19066582</ref>. <br /> |
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| ==3D structures of resolvase== | | ==3D structures of resolvase== |
| | [[Resolvase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| * Holliday junction resolvase
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| **[[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br />
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| **[[2wcw]], [[2wcz]] – AfHJR (mutant)<br />
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| **[[2h8c]] – EcHJR RusA (mutant) + DNA – ''Escherichia coli''<br />
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| **[[1gdt]] - EcHJR +DNA<br />
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| **[[2h8e]] - EcHJR RusA (mutant)<br />
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| **[[1q8r]] – EcHJR RusA<br />
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| **[[1hjr]] – EcHJR RuvC<br />
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| **[[4ep4]] – TtHJR RuvC – ''Thermus thermophilus''<br />
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| **[[4ep5]] – TtHJR RuvC (mutant)<br />
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| **[[1zp7]] – HJR RecU – ''Bacillus subtilis''<br />
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| **[[1ob8]], [[1ob9]], [[1hh1]] – SsHJR – ''Sulfolobus solfataricus''<br />
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| **[[2fco]] – HJR – ''Geobacillus kaustophilus''<br />
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| **[[1ipi]], [[1gef]] – HJR – ''Pyrococcus furiosus''<br />
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| **[[1kcf]] – HJR – fission yeast<br />
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| * Ser-recombinase
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| **[[2rsl]] - EcGDR<br />
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| **[[1hx7]] – EcGDR N-terminal – NMR<br />
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| **[[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br />
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| **[[1gdr]], [[1hx7]] - EcGDR catalytic domain<br />
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| **[[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br />
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| **[[2gm5]] – EcGDR (mutant)<br />
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| **[[3guv]] – site-specific recombinase – ''Streptococcus pneumoniae''<br />
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| **[[2r0q]] – S-rec+DNA – ''Staphylococcus aureus''<br />
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| **[[3lhf]] – SsS-rec<br />
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| * Tyr-recombinase
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| **[[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br />
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| **[[2b21]] - MmRadA Rec+AMPPNP<br />
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| **[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br />
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| **[[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br />
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| **[[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br />
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| **[[2f1i]] – MvRec+AMPPNP<br />
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| **[[2f1j]], [[2fpk]] - MvRec+ADP<br />
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| **[[1a0p]] – EcXerd Rec<br />
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| * Cre recombinase
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| **[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br />
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| *Hin recombinase
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| **[[1hcr]], [[1ijw]], [[1ijj6]], [[1jj8]], [[1jko]], [[1jkp]], [[1jkq]], [[1jkr]] – HRec + DNA – synthetic <br />
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| *RadA recombinase
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| **[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA <br />
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| **[[4dc9]] – MvRadA C-terminal domain <br />
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| **[[2i1q]] – MvRadA (mutant) <br />
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| **[[1pzn]] – PfRadA <br />
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| **[[4a6p]] – PfRadA ATPase domain (mutant) <br />
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| *RadA recombinase complex
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| **[[5fot]], [[5fou]], [[5fov]], [[5fow]], [[5fpk]], [[4b3b]] – PfRadA ATPase domain + tetrapeptide <br />
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| **[[4d6p]] – PfRadA ATPase domain (mutant) + AMPPNP <br />
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| **[[4uqo]] – PfRadA ATPase domain (mutant) + ADP <br />
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| **[[4b2i]] – PfRadA ATPase domain (mutant) + indazole <br />
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| **[[4b2l]], [[4b35]] – PfRadA ATPase domain (mutant) + methylester tryplophan <br />
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| **[[4b32]], [[4b33]] – PfRadA ATPase domain (mutant) + naphthol <br />
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| **[[4b34]] – PfRadA ATPase domain (mutant) + aminobenzothiazole <br />
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| **[[4b3c]], [[4b3d]] – PfRadA ATPase domain (mutant) + indole <br />
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| **[[4a6x]] – PfRadA ATPase domain (mutant) + ATP <br />
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| **[[3ntu]], [[2gdj]] – MvRadA + AMPPNP <br />
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| **[[2b21]], [[2f1h]], [[2f1i]], [[2fpl]], [[2fpm]] – MvRadA (mutant) + AMPPNP <br />
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| **[[3fyh]], [[2f1j]], [[2fpk]] – MvRadA (mutant) + ADP <br />
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| **[[3etl]], [[3ew9]], [[3ewa]] – MmRadA (mutant) + AMPPNP <br />
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| *RadB recombinase
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| **[[2cvf]], [[2cvh]] – TkRadB <br />
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| * Integrase
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| **[[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br />
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| **[[3bvp]] – Integrase N-terminal – Lactococcus phage<br />
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| **[[2oxo]] – LamInt binding domain – phage<br />
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| **[[1z19]] – EpLamInt core binding +catalytic domain+DNA<br />
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| **[[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br />
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| **[[1kjk]] – EpLamInt N-terminal – NMR<br />
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| **[[1ae9]] – EpLamInt catalytic core<br />
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| **[[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br />
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| **[[1flo]] - yFLP +DNA<br />
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| **[[1aih]] – HP1 integrase catalytic domain - bacteriophage<br />
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| **[[3nrw]] – INT/site-specific recombinase N-terminal – ''Haloarcula marismortui''<br />
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| **[[3jtz]] – INT arm-type binding domain – ''Yersinia pestis''<br />
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| **[[3ju0]] – HPI INT arm-type binding domain – ''Pectobacterium atrosepticum''<br />
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| **[[2kkp]] – INT SAM-like domain – ''Moorella thermoacetica'' – NMR<br />
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| **[[2kkv]] – INT fragment – ''Salmonella enterica'' – NMR<br />
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| **[[2khq]] - INT fragment – ''Staphylococcus saprophyticus'' – NMR<br />
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| **[[2wcc]] – λINT DBD+ DNA – Enterobacteria phage λ - NMR<br />
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| **[[2khv]], [[2kj5]] – INT residues 102-199 – ''Nitrosospira multiformis'' – NMR<br />
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| *Recombinase A (RecA) see [[Recombinase A]]
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| }}
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| ==References== | | ==References== |
| <references /> | | <references /> |
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| [[Category:Topic Page]] | | [[Category:Topic Page]] |
