5lz0: Difference between revisions
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==Llama nanobody PorM_01== | |||
<StructureSection load='5lz0' size='340' side='right'caption='[[5lz0]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5lz0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LZ0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lz0 OCA], [https://pdbe.org/5lz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lz0 RCSB], [https://www.ebi.ac.uk/pdbsum/5lz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lz0 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM. | |||
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.,Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi:, 10.1107/S2053230X17005969. Epub 2017 Apr 26. PMID:28471361<ref>PMID:28471361</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5lz0" style="background-color:#fffaf0;"></div> | ||
[[Category: Duhoo | == References == | ||
[[Category: Leone | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lama glama]] | |||
[[Category: Large Structures]] | |||
[[Category: Duhoo Y]] | |||
[[Category: Leone P]] | |||
[[Category: Roussel A]] | |||
