5mji: Difference between revisions
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==Crystal Structure of RosB with bound intermediate OHC-RP (8-demethyl-8-formylriboflavin-5'-phosphate)== | |||
<StructureSection load='5mji' size='340' side='right' caption='[[5mji]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5mji]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MJI FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7O6:[(2~{S},3~{R},4~{R})-5-[8-METHANOYL-7-METHYL-2,4-BIS(OXIDANYLIDENE)-1~{H}-BENZO[G]PTERIDIN-10-IUM-10-YL]-2,3,4-TRIS(OXIDANYL)PENTYL]+DIHYDROGEN+PHOSPHATE'>7O6</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mji OCA], [http://pdbe.org/5mji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mji RCSB], [http://www.ebi.ac.uk/pdbsum/5mji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mji ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
8-demethyl-8-aminoriboflavin-5'-phosphate (AFP) synthase (RosB) catalyzes the key reaction of roseoflavin biosynthesis by forming AFP from riboflavin-5'-phosphate (RP) and glutamate via the intermediates 8-demethyl-8-formylriboflavin-5'-phosphate (OHC-RP) and 8-demethyl-8-carboxylriboflavin-5'-phosphate (HO2 C-RP). To understand this reaction in which a methyl substituent of an aromatic ring is replaced by an amine we structurally characterized RosB in complex with OHC-RP (2.0 A) and AFP (1.7 A). RosB is composed of four flavodoxin-like subunits which have been upgraded with specific extensions and a unique C-terminal arm. It appears that RosB has evolved from an electron- or hydride-transferring flavoprotein to a sophisticated multi-step enzyme which uses RP as a substrate (and not as a cofactor). Structure-based active site analysis was complemented by mutational and isotope-based mass-spectrometric data to propose an enzymatic mechanism on an atomic basis. | |||
The Crystal Structure of RosB: Insights into the Reaction Mechanism of the First Member of a Family of Flavodoxin-like Enzymes.,Konjik V, Brunle S, Demmer U, Vanselow A, Sandhoff R, Ermler U, Mack M Angew Chem Int Ed Engl. 2016 Dec 16. doi: 10.1002/anie.201610292. PMID:27981706<ref>PMID:27981706</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5mji" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bruenle, S]] | |||
[[Category: Demmer, U]] | |||
[[Category: Ermler, U]] | |||
[[Category: Konjik, V]] | |||
[[Category: Mack, M]] | |||
[[Category: Sandhoff, R]] | |||
[[Category: Vanselow, A]] | |||
[[Category: Electron transport]] | |||
[[Category: Flavodoxin-like]] | |||
[[Category: Flavoprotein]] | |||
[[Category: Roseoflavin]] | |||
