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New page: '''Unreleased structure''' The entry 5tsb is ON HOLD until Paper Publication Authors: Zhang, T., Fellner, M., Sui, D., Hu, J. Description: Crystal Structure of a metal binding protein ...
 
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'''Unreleased structure'''


The entry 5tsb is ON HOLD  until Paper Publication
==Crystal structure of the Zrt-/Irt-like protein from Bordetella bronchiseptica with bound Cd2+==
<StructureSection load='5tsb' size='340' side='right'caption='[[5tsb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5tsb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_bronchiseptica_RB50 Bordetella bronchiseptica RB50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TSB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tsb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsb OCA], [https://pdbe.org/5tsb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tsb RCSB], [https://www.ebi.ac.uk/pdbsum/5tsb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ZIP_BORBR ZIP_BORBR] Selective electrodiffusional channel that mediates the uptake of Zn(2+). Exploits in vivo zinc concentration gradients (maintained by cellular zinc homeostasis) to passively move zinc ions into the cytoplasm. ZIPB-mediated zinc flux is dependent upon pH, but independent of the proton motive force. Is also able to import Cd(2+), but is not permeable to Co(2+), Cu(2+), Fe(2+), Mn(2+) and Ni(2+).<ref>PMID:20876577</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Zrt/Irt-like proteins (ZIPs) play fundamental roles in metal metabolism/homeostasis and are broadly involved in numerous physiological and pathological processes. The lack of high-resolution structure of the ZIPs hinders understanding of the metal transport mechanism. We report two crystal structures of a prokaryotic ZIP in lipidic cubic phase with bound metal substrates (Cd2+ at 2.7 A and Zn2+ at 2.4 A). The structures revealed a novel 3+2+3TM architecture and an inward-open conformation occluded at the extracellular side. Two metal ions were trapped halfway through the membrane, unexpectedly forming a binuclear metal center. The Zn2+-substituted structure suggested asymmetric functions of the two metal-binding sites and also revealed a route for zinc release. Mapping of disease-causing mutations, structure-guided mutagenesis, and cell-based zinc transport assay demonstrated the crucial role of the binuclear metal center for human ZIP4. A metal transport mechanism for the ZIP from Bordetella bronchiseptica was proposed, which is likely applicable to other ZIPs.


Authors: Zhang, T., Fellner, M., Sui, D., Hu, J.
Crystal structures of a ZIP zinc transporter reveal a binuclear metal center in the transport pathway.,Zhang T, Liu J, Fellner M, Zhang C, Sui D, Hu J Sci Adv. 2017 Aug 25;3(8):e1700344. doi: 10.1126/sciadv.1700344. eCollection 2017, Aug. PMID:28875161<ref>PMID:28875161</ref>


Description: Crystal Structure of a metal binding protein
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Zhang, T]]
<div class="pdbe-citations 5tsb" style="background-color:#fffaf0;"></div>
[[Category: Fellner, M]]
== References ==
[[Category: Sui, D]]
<references/>
[[Category: Hu, J]]
__TOC__
</StructureSection>
[[Category: Bordetella bronchiseptica RB50]]
[[Category: Large Structures]]
[[Category: Fellner M]]
[[Category: Hu J]]
[[Category: Liu J]]
[[Category: Sui D]]
[[Category: Zhang T]]
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