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==MOLECULAR BASIS FOR COHESIN ACETYLATION BY ESTABLISHMENT OF SISTER CHROMATID COHESION N-ACETYLTRANSFERASE ESCO1==
==MOLECULAR BASIS FOR COHESIN ACETYLATION BY ESTABLISHMENT OF SISTER CHROMATID COHESION N-ACETYLTRANSFERASE ESCO1==
<StructureSection load='5t53' size='340' side='right' caption='[[5t53]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='5t53' size='340' side='right'caption='[[5t53]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5t53]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T53 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T53 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5t53]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T53 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.699&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t53 OCA], [http://pdbe.org/5t53 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t53 RCSB], [http://www.ebi.ac.uk/pdbsum/5t53 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t53 ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t53 OCA], [https://pdbe.org/5t53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t53 RCSB], [https://www.ebi.ac.uk/pdbsum/5t53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t53 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/ESCO1_HUMAN ESCO1_HUMAN]] Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.<ref>PMID:14576321</ref> <ref>PMID:15958495</ref> <ref>PMID:18614053</ref> <ref>PMID:19907496</ref> 
[https://www.uniprot.org/uniprot/ESCO1_HUMAN ESCO1_HUMAN] Acetyltransferase required for the establishment of sister chromatid cohesion and couple the processes of cohesion and DNA replication to ensure that only sister chromatids become paired together. In contrast to the structural cohesins, the deposition and establishment factors are required only during S phase. Acts by mediating the acetylation of cohesin component SMC3.<ref>PMID:14576321</ref> <ref>PMID:15958495</ref> <ref>PMID:18614053</ref> <ref>PMID:19907496</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein acetylation is a prevalent posttranslational modification that is regulated by diverse acetyltransferase enzymes. While histone acetyltransferases (HATs) have been well characterized both structurally and mechanistically, far less is known about non-histone acetyltransferase enzymes. The human ESCO1 and ESCO2 paralogs acetylate the cohesin complex subunit SMC3 to regulate the separation of sister chromatids during mitosis and meiosis. Missense mutations within the acetyltransferase domain of these proteins correlate with diseases, including endometrial cancers and Roberts Syndrome. Despite their biological importance, the mechanisms underlying acetylation by the ESCO proteins are not understood. Here, we report the X-ray crystal structure of the highly conserved zinc finger-acetyltransferase moiety of ESCO1 with accompanying structure-based mutagenesis and biochemical characterization. We find that the ESCO1acetyltransferase core is structurally homologous to the Gcn5 HAT, but contains unique additional features including a zinc finger and a ~40-residue loop region that appear to play roles in protein stability and SMC3 substrate binding. We identify key residues that play roles in substrate binding and catalysis, and rationalize the functional consequences of disease-associated mutations. Together, these studies reveal the molecular basis for SMC3 acetylation by ESCO1 and have broader implications for understanding the structure/function of non-histone acetyltransferases.
 
Molecular Basis for Cohesin Acetylation by Establishment of Sister Chromatid Cohesion N-acetyltransferase ESCO1.,Rivera-Colon Y, Maguire A, Liszczak GP, Olia AS, Marmorstein R J Biol Chem. 2016 Nov 1. pii: jbc.M116.752220. PMID:27803161<ref>PMID:27803161</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5t53" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Liszczak, G P]]
[[Category: Homo sapiens]]
[[Category: Maguire, A]]
[[Category: Large Structures]]
[[Category: Marmorstein, R]]
[[Category: Liszczak GP]]
[[Category: Olia, A S]]
[[Category: Maguire A]]
[[Category: Rivera-Colon, Y]]
[[Category: Marmorstein R]]
[[Category: Acetyltransferase]]
[[Category: Olia AS]]
[[Category: Cohesin]]
[[Category: Rivera-Colon Y]]
[[Category: Esco1]]
[[Category: Smc3]]
[[Category: Transferase]]
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