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==The structure of monooxygenase KstA11 in the biosynthetic pathway of kosinostatin==
==The structure of monooxygenase KstA11 in the biosynthetic pathway of kosinostatin==
<StructureSection load='5f5l' size='340' side='right' caption='[[5f5l]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
<StructureSection load='5f5l' size='340' side='right'caption='[[5f5l]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5f5l]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F5L FirstGlance]. <br>
<table><tr><td colspan='2'>[[5f5l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_sp._TP-A0468 Micromonospora sp. TP-A0468]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F5L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f5n|5f5n]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5l OCA], [https://pdbe.org/5f5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f5l RCSB], [https://www.ebi.ac.uk/pdbsum/5f5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5l ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5l OCA], [http://pdbe.org/5f5l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5l RCSB], [http://www.ebi.ac.uk/pdbsum/5f5l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5l ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A023GUL3_9ACTN A0A023GUL3_9ACTN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ranking among the most effective anticancer drugs, anthracyclines represent an important family of aromatic polyketides generated by type II polyketide synthases (PKSs). After formation of polyketide cores, the post-PKS tailoring modifications endow the scaffold with various structural diversities and biological activities. Here we demonstrate an unprecedented four-enzyme-participated hydroxyl regioisomerization process involved in the biosynthesis of kosinostatin. First, KstA15 and KstA16 function together to catalyze a cryptic hydroxylation of the 4-hydroxyl-anthraquinone core, yielding a 1,4-dihydroxyl product, which undergoes a chemically challenging asymmetric reduction-dearomatization subsequently acted by KstA11; then, KstA10 catalyzes a region-specific reduction concomitant with dehydration to afford the 1-hydroxyl anthraquinone. Remarkably, the shunt product identifications of both hydroxylation and reduction-dehydration reactions, the crystal structure of KstA11 with bound substrate and cofactor, and isotope incorporation experiments reveal mechanistic insights into the redox dearomatization and rearomatization steps. These findings provide a distinguished tailoring paradigm for type II PKS engineering.
Hydroxyl regioisomerization of anthracycline catalyzed by a four-enzyme cascade.,Zhang Z, Gong YK, Zhou Q, Hu Y, Ma HM, Chen YS, Igarashi Y, Pan L, Tang GL Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1554-1559. doi:, 10.1073/pnas.1610097114. Epub 2017 Jan 30. PMID:28137838<ref>PMID:28137838</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5f5l" style="background-color:#fffaf0;"></div>
==See Also==
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Gong, Y]]
[[Category: Large Structures]]
[[Category: Pan, L]]
[[Category: Micromonospora sp. TP-A0468]]
[[Category: Dearomatization]]
[[Category: Gong Y]]
[[Category: Kosinostatin]]
[[Category: Pan L]]
[[Category: Monooxygenase]]
[[Category: Oxidoreductase]]
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