1r44: Difference between revisions

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[[Image:1r44.jpg|left|200px]]


{{Structure
==Crystal Structure of VanX==
|PDB= 1r44 |SIZE=350|CAPTION= <scene name='initialview01'>1r44</scene>, resolution 2.25&Aring;
<StructureSection load='1r44' size='340' side='right'caption='[[1r44]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
<table><tr><td colspan='2'>[[1r44]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R44 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R44 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
|GENE= VANX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1352 Enterococcus faecium])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r44 OCA], [https://pdbe.org/1r44 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r44 RCSB], [https://www.ebi.ac.uk/pdbsum/1r44 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r44 ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r44 OCA], [http://www.ebi.ac.uk/pdbsum/1r44 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r44 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/VANX_ENTFC VANX_ENTFC] Catalyzes hydrolysis of the D-alanyl-D-alanine dipeptide.
 
== Evolutionary Conservation ==
'''Crystal Structure of VanX'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r4/1r44_consurf.spt"</scriptWhenChecked>
VanX is a zinc-dependent D-alanyl-D-alanine dipeptidase that is a critical component in a system that mediates transposon-based vancomycin resistance in enterococci. It is also a key drug target in circumventing clinical vancomycin resistance. The structure of VanX from E. faecium has been solved by X-ray crystallography and reveals a Zn(2+)-dipeptidase with a unique overall fold and a well-defined active site confined within a cavity of limited size. The crystal structures of VanX, the VanX:D-alanyl-D-alanine complex, the VanX:D-alanine complex, and VanX in complex with phosphonate and phosphinate transition-state analog inhibitors, are also presented at high resolution. Structural homology searches of known structures revealed that the fold of VanX is similar to those of two proteins: the N-terminal fragment of murine Sonic hedgehog and the Zn(2+)-dependent N-acyl-D-alanyl-D-alanine carboxypeptidase of S. albus G.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1R44 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R44 OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r44 ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance., Bussiere DE, Pratt SD, Katz L, Severin JM, Holzman T, Park CH, Mol Cell. 1998 Jul;2(1):75-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9702193 9702193]
</StructureSection>
[[Category: Enterococcus faecium]]
[[Category: Enterococcus faecium]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Holzman, T.]]
[[Category: Holzman T]]
[[Category: Katz, L.]]
[[Category: Katz L]]
[[Category: Park, C H.]]
[[Category: Park CH]]
[[Category: Pratt, S D.]]
[[Category: Pratt SD]]
[[Category: Severin, J M.]]
[[Category: Severin JM]]
[[Category: dipeptidase]]
[[Category: e faecium]]
[[Category: vanx]]
 
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Retrieved from "https://proteopedia.org/1r44"