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[[Image:1r5m.gif|left|200px]]


{{Structure
==Crystal Structure Of The C-Terminal WD40 Domain Of Sif2==
|PDB= 1r5m |SIZE=350|CAPTION= <scene name='initialview01'>1r5m</scene>, resolution 1.55&Aring;
<StructureSection load='1r5m' size='340' side='right'caption='[[1r5m]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
<table><tr><td colspan='2'>[[1r5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5M FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
|GENE= SIF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5m OCA], [https://pdbe.org/1r5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5m RCSB], [https://www.ebi.ac.uk/pdbsum/1r5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5m ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5m OCA], [http://www.ebi.ac.uk/pdbsum/1r5m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r5m RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/SIF2_YEAST SIF2_YEAST] Antagonizes telomeric silencing in yeast. May recruit SIR4 to non-telomeric sites or repression.
 
== Evolutionary Conservation ==
'''Crystal Structure Of The C-Terminal WD40 Domain Of Sif2'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/1r5m_consurf.spt"</scriptWhenChecked>
In Saccharomyces cerevisiae, the SIF2 gene product is an integral component of the Set3 complex (SET3C), an assembly of proteins with some homology to the human SMRT and N-CoR corepressor complexes. SET3C has histone deacetylase activity that is responsible for repressing a set of meiotic genes. We have determined the X-ray crystal structure of a 46 kDa C-terminal domain of a SET3C core protein, Sif2p to 1.55 A resolution and a crystallographic R-factor of 19.0%. This domain contains an unusual eight-bladed beta-propeller structure, which differs from other transcriptional corepressor structures such as yeast Tup1p and human groucho (Gro)/TLE1, which have only seven. We have demonstrated intact Sif2p is a tetramer and the N-terminal LisH (Lis-homology)-containing domain mediates tetramerization and interaction with another component of SET3C, Snt1p. Multiple sequence alignments indicate that a surface on the "top" of the protein is conserved among species, suggesting that it may play a common role in binding partner proteins. Since Sif2p appears to be the yeast homolog of human TBL1 and TBLR1, which function in the N-CoR/SMRT complexes, its structural and oligomeric properties are likely to be very similar.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1R5M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5M OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5m ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
The structure of Sif2p, a WD repeat protein functioning in the SET3 corepressor complex., Cerna D, Wilson DK, J Mol Biol. 2005 Aug 26;351(4):923-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16051270 16051270]
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Cerna D]]
[[Category: Cerna, D.]]
[[Category: Wilson DK]]
[[Category: Wilson, D K.]]
[[Category: beta propeller]]
[[Category: transcription corepressor]]
[[Category: wd40 repeat]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:40 2008''

Latest revision as of 08:20, 14 February 2024

Crystal Structure Of The C-Terminal WD40 Domain Of Sif2

1r5m, resolution 1.55Å

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