OBrochtasSandbox: Difference between revisions
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New page: ==O'Brochta's Sandbox == 0 <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> This is a default text for your page '''OBrochtasSandbox'''.... |
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== | ==Anopheles arabiensis Insulin Receptor like Protein== | ||
<StructureSection load=' | <StructureSection load='Model.itasser.relax.pdb' side='right' caption='Anopheles arabiensis InR kinase domain model' scene='75/750300/Kinase_n_and_c_lobes/1'> | ||
The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project' | |||
This shows the basic structure of the kinase domain with an <scene name='75/750300/Kinase_n_and_c_lobes/1'>N and C lobe</scene>. The ATP will sit between them just below Beta 1 | |||
<scene name='75/750300/Inr_atp-cat_loop/3'>This</scene> shows the ATP binding site (purple) and the catalytic loop (green). | |||
Here it is as a <scene name='75/750300/Inr_atp-cat_loopspacefill/1'>spacefilled model</scene>. | |||
These are <scene name='75/750300/Geneticmutations/1'>three ts mutations</scene> found in other RTKs. | |||
G1000S (magenta), A1133G (orange), T1221I (teal) | |||
And as a <scene name='75/750300/Geneticmutationsspacefilled/1'>spacefilled model</scene> | |||
This <scene name='75/750300/T1221_spacefilled/1'>threonine</scene> when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster | |||
Using the software TS_pred (a ts mutation prediction program): these are the <scene name='75/750300/Geneticmutations-tspred_rosett/2'>5 best sites</scene> to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red) | |||
Using Rosetta_ts_rbf (another ts mutation prediction program): these are the <scene name='75/750300/Geneticmutations-tspred_rosett/2'>top 5</scene> sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow) | |||
Both TS_pred and Rosetta_ts_rbf had <scene name='75/750300/Tspredrosetta_spacefilled/1'>L1197</scene> in their top 5 (black) | |||
Most but not all of the actual and predicted mutations are <scene name='75/750300/Tspred_rosett_burried_residues/1'>buried</scene>. Note that T1221 and G1000S are not buried. | |||
Here are the <scene name='75/750300/Tspred_rosett_hydroph_residues/1'>hydrophobic residues</scene>. | |||
</StructureSection> | |||
