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Anopheles arabiensis Insulin Receptor like Protein

spinqualitylabelsall models Anopheles arabiensis InR kinase domain model Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project' This shows the basic structure of the kinase domain with an N and C lobe. The ATP will sit between them just below Beta 1 This shows the ATP binding site (purple) and the catalytic loop (green). Here it is as a spacefilled model. These are three ts mutations found in other RTKs. G1000S (magenta), A1133G (orange), T1221I (teal) And as a spacefilled model This threonine when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster Using the software TS_pred (a ts mutation prediction program): these are the 5 best sites to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red) Using Rosetta_ts_rbf (another ts mutation prediction program): these are the top 5 sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow) Both TS_pred and Rosetta_ts_rbf had L1197 in their top 5 (black) Most but not all of the actual and predicted mutations are buried. Note that T1221 and G1000S are not buried. Here are the hydrophobic residues.

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David O'Brochta