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==Structure of Uranotaenia sapphirina cypovirus (CPV17) polyhedrin at 100 K==
==Structure of Uranotaenia sapphirina cypovirus (CPV17) polyhedrin at 100 K==
<StructureSection load='4s1k' size='340' side='right' caption='[[4s1k]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='4s1k' size='340' side='right'caption='[[4s1k]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4s1k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uranotaenia_sapphirina_cypovirus Uranotaenia sapphirina cypovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4S1K FirstGlance]. <br>
<table><tr><td colspan='2'>[[4s1k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uranotaenia_sapphirina_cypovirus Uranotaenia sapphirina cypovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S1K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S1K FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4s1l|4s1l]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4s1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s1k OCA], [http://pdbe.org/4s1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4s1k RCSB], [http://www.ebi.ac.uk/pdbsum/4s1k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4s1k ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s1k OCA], [https://pdbe.org/4s1k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s1k RCSB], [https://www.ebi.ac.uk/pdbsum/4s1k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s1k ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q5EK29_9VIRU Q5EK29_9VIRU]
The X-ray free-electron laser (XFEL) allows the analysis of small weakly diffracting protein crystals, but has required very many crystals to obtain good data. Here we use an XFEL to determine the room temperature atomic structure for the smallest cytoplasmic polyhedrosis virus polyhedra yet characterized, which we failed to solve at a synchrotron. These protein microcrystals, roughly a micron across, accrue within infected cells. We use a new physical model for XFEL diffraction, which better estimates the experimental signal, delivering a high-resolution XFEL structure (1.75 A), using fewer crystals than previously required for this resolution. The crystal lattice and protein core are conserved compared with a polyhedrin with less than 10% sequence identity. We explain how the conserved biological phenotype, the crystal lattice, is maintained in the face of extreme environmental challenge and massive evolutionary divergence. Our improved methods should open up more challenging biological samples to XFEL analysis.


Structure of CPV17 polyhedrin determined by the improved analysis of serial femtosecond crystallographic data.,Ginn HM, Messerschmidt M, Ji X, Zhang H, Axford D, Gildea RJ, Winter G, Brewster AS, Hattne J, Wagner A, Grimes JM, Evans G, Sauter NK, Sutton G, Stuart DI Nat Commun. 2015 Mar 9;6:6435. doi: 10.1038/ncomms7435. PMID:25751308<ref>PMID:25751308</ref>
==See Also==
 
*[[Polyhedrin|Polyhedrin]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4s1k" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Uranotaenia sapphirina cypovirus]]
[[Category: Uranotaenia sapphirina cypovirus]]
[[Category: Axford, D]]
[[Category: Axford D]]
[[Category: Brewster, A S]]
[[Category: Brewster AS]]
[[Category: Evans, G]]
[[Category: Evans G]]
[[Category: Gildea, R J]]
[[Category: Gildea RJ]]
[[Category: Ginn, H M]]
[[Category: Ginn HM]]
[[Category: Grimes, J M]]
[[Category: Grimes JM]]
[[Category: Hattne, J]]
[[Category: Hattne J]]
[[Category: Ji, X]]
[[Category: Ji X]]
[[Category: Messerschmidt, M]]
[[Category: Messerschmidt M]]
[[Category: Sauter, N K]]
[[Category: Sauter NK]]
[[Category: Stuart, D I]]
[[Category: Stuart DI]]
[[Category: Sutton, G]]
[[Category: Sutton G]]
[[Category: Wagner, A]]
[[Category: Wagner A]]
[[Category: Winter, G]]
[[Category: Winter G]]
[[Category: Zhang, H]]
[[Category: Zhang H]]
[[Category: Polyhedrin]]
[[Category: Viral protein]]

Latest revision as of 12:57, 1 March 2024

Structure of Uranotaenia sapphirina cypovirus (CPV17) polyhedrin at 100 K

4s1k, resolution 2.20Å

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