5muv: Difference between revisions

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New page: '''Unreleased structure''' The entry 5muv is ON HOLD until Paper Publication Authors: Sun, Z., El Omari, K., Sun, X., Ilca, S., Kotecha, A., Stuart, D.I., Poranen, M.M., Huiskonen, J.T....
 
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'''Unreleased structure'''


The entry 5muv is ON HOLD  until Paper Publication
==Atomic structure fitted into a localized reconstruction of bacteriophage phi6 packaging hexamer P4==
<SX load='5muv' size='340' side='right' viewer='molstar' caption='[[5muv]], [[Resolution|resolution]] 9.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5muv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_phi6 Pseudomonas virus phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MUV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MUV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5muv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5muv OCA], [https://pdbe.org/5muv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5muv RCSB], [https://www.ebi.ac.uk/pdbsum/5muv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5muv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/P4_BPPH6 P4_BPPH6] P4 is one of the structural proteins of the polyhedral procapsid, which is responsible for genomic replication and transcription. Displays single-stranded RNA-stimulated NTPase activity.<ref>PMID:12239286</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Correct outer protein shell assembly is a prerequisite for virion infectivity in many multi-shelled dsRNA viruses. In the prototypic dsRNA bacteriophage phi6, the assembly reaction is promoted by calcium ions but its biomechanics remain poorly understood. Here, we describe the near-atomic resolution structure of the phi6 double-shelled particle. The outer T=13 shell protein P8 consists of two alpha-helical domains joined by a linker, which allows the trimer to adopt either a closed or an open conformation. The trimers in an open conformation swap domains with each other. Our observations allow us to propose a mechanistic model for calcium concentration regulated outer shell assembly. Furthermore, the structure provides a prime exemplar of bona fide domain-swapping. This leads us to extend the theory of domain-swapping from the level of monomeric subunits and multimers to closed spherical shells, and to hypothesize a mechanism by which closed protein shells may arise in evolution.


Authors: Sun, Z., El Omari, K., Sun, X., Ilca, S., Kotecha, A., Stuart, D.I., Poranen, M.M., Huiskonen, J.T.
Double-stranded RNA virus outer shell assembly by bona fide domain-swapping.,Sun Z, El Omari K, Sun X, Ilca SL, Kotecha A, Stuart DI, Poranen MM, Huiskonen JT Nat Commun. 2017 Mar 13;8:14814. doi: 10.1038/ncomms14814. PMID:28287099<ref>PMID:28287099</ref>


Description: Atomic structure fitted into a localized reconstruction of bacteriophage phi6 packaging hexamer P4
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Poranen, M.M]]
<div class="pdbe-citations 5muv" style="background-color:#fffaf0;"></div>
[[Category: Kotecha, A]]
== References ==
[[Category: Sun, Z]]
<references/>
[[Category: El Omari, K]]
__TOC__
[[Category: Huiskonen, J.T]]
</SX>
[[Category: Ilca, S]]
[[Category: Large Structures]]
[[Category: Stuart, D.I]]
[[Category: Pseudomonas virus phi6]]
[[Category: Sun, X]]
[[Category: El Omari K]]
[[Category: Huiskonen JT]]
[[Category: Ilca S]]
[[Category: Kotecha A]]
[[Category: Poranen MM]]
[[Category: Stuart DI]]
[[Category: Sun X]]
[[Category: Sun Z]]
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