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==CryoEM structure of type II secretion system secretin GspD in E.coli K12==
==CryoEM structure of type II secretion system secretin GspD in E.coli K12==
<StructureSection load='5wq7' size='340' side='right' caption='[[5wq7]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
<SX load='5wq7' size='340' side='right' viewer='molstar' caption='[[5wq7]], [[Resolution|resolution]] 3.04&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5wq7]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WQ7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5wq7]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WQ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WQ7 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wq8|5wq8]], [[5wq9|5wq9]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.04&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq7 OCA], [http://pdbe.org/5wq7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wq7 RCSB], [http://www.ebi.ac.uk/pdbsum/5wq7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wq7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wq7 OCA], [https://pdbe.org/5wq7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wq7 RCSB], [https://www.ebi.ac.uk/pdbsum/5wq7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wq7 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/GSPD_ECOLI GSPD_ECOLI]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.  
[https://www.uniprot.org/uniprot/GSPD_ECOLI GSPD_ECOLI] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The secretin GspD of the type II secretion system (T2SS) forms a channel across the outer membrane in Gram-negative bacteria to transport substrates from the periplasm to the extracellular milieu. The lack of an atomic-resolution structure of the GspD channel hinders the investigation of substrate translocation mechanism of T2SS. Here we report cryo-EM structures of two GspD channels ( approximately 1 MDa), from Escherichia coli K12 and Vibrio cholerae, at approximately 3 A resolution. The structures reveal a pentadecameric channel architecture, wherein three rings of GspD N domains form the periplasmic channel. The secretin domain constitutes a novel double beta-barrel channel, with at least 60 beta-strands in each barrel, and is stabilized by S domains. The outer membrane channel is sealed by beta-strand-enriched gates. On the basis of the partially open state captured, we proposed a detailed gate-opening mechanism. Our structures provide a structural basis for understanding the secretin superfamily and the mechanism of substrate translocation in T2SS.
 
Structural insights into the secretin translocation channel in the type II secretion system.,Yan Z, Yin M, Xu D, Zhu Y, Li X Nat Struct Mol Biol. 2017 Feb;24(2):177-183. doi: 10.1038/nsmb.3350. Epub 2017, Jan 9. PMID:28067918<ref>PMID:28067918</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5wq7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Li, X]]
[[Category: Escherichia coli K-12]]
[[Category: Yan, Z]]
[[Category: Large Structures]]
[[Category: Yin, M]]
[[Category: Li X]]
[[Category: C15 symmetry]]
[[Category: Yan Z]]
[[Category: Protein transport]]
[[Category: Yin M]]
[[Category: Secretin family]]
[[Category: T2ss]]
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