5mvu: Difference between revisions
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==Crystal structure of an A-DNA dodecamer containing 5-formylcytosine in 3 consecutive CpG steps== | |||
<StructureSection load='5mvu' size='340' side='right'caption='[[5mvu]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5mvu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MVU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5FC:5-FORMYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5FC</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mvu OCA], [https://pdbe.org/5mvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mvu RCSB], [https://www.ebi.ac.uk/pdbsum/5mvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mvu ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The mechanism by which the recently identified DNA modification 5-formylcytosine (fC) is recognized by epigenetic writer and reader proteins is not known. Recently, an unusual DNA structure, F-DNA, has been proposed as the basis for enzyme recognition of clusters of fC. We used NMR and X-ray crystallography to compare several modified DNA duplexes with unmodified analogs and found that in the crystal state the duplexes all belong to the A family, whereas in solution they are all members of the B family. We found that, contrary to previous findings, fC does not significantly affect the structure of DNA, although there are modest local differences at the modification sites. Hence, global conformation changes are unlikely to account for the recognition of this modified base, and our structural data favor a mechanism that operates at base-pair resolution for the recognition of fC by epigenome-modifying enzymes. | |||
5-Formylcytosine does not change the global structure of DNA.,Hardwick JS, Ptchelkine D, El-Sagheer AH, Tear I, Singleton D, Phillips SEV, Lane AN, Brown T Nat Struct Mol Biol. 2017 May 15. doi: 10.1038/nsmb.3411. PMID:28504696<ref>PMID:28504696</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5mvu" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Brown T]] | |||
[[Category: Hardwick JS]] | |||
[[Category: Phillips SEV]] | |||
[[Category: Ptchelkine D]] | |||
Latest revision as of 17:51, 8 November 2023
Crystal structure of an A-DNA dodecamer containing 5-formylcytosine in 3 consecutive CpG steps
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