5n76: Difference between revisions
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New page: '''Unreleased structure''' The entry 5n76 is ON HOLD Authors: Timm, J., Brochier-Armanet, C., Perard, J., Zambelli, B., Ollagnier-de-Choudens, S., Ciurli, S., Cavazza, C. Description: ... |
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==Crystal structure of the apo-form of the CO dehydrogenase accessory protein CooT from Rhodospirillum rubrum== | |||
<StructureSection load='5n76' size='340' side='right'caption='[[5n76]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5n76]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N76 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N76 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n76 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n76 OCA], [https://pdbe.org/5n76 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n76 RCSB], [https://www.ebi.ac.uk/pdbsum/5n76 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n76 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/P72320_RHORU P72320_RHORU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In Rhodospirillum rubrum, maturation of Carbon Monoxide Dehydrogenase (CODH) requires three accessory proteins, CooC, CooT and CooJ, dedicated to nickel insertion into the active site, which is constituted by a distorted [NiFe3S4] cubane coordinated with a mononuclear Fe site. CooC is an ATPase proposed to provide the energy required for the maturation process, while CooJ is described as a metallochaperone with 16 histidines and 2 cysteines at the C-terminus, likely involved in metal binding and/or storage. Prior to the present study, no information was available on CooT at the molecular level. Here, the X-ray structure of RrCooT was obtained, which revealed that this protein is a homodimer featuring a fold that resembles an Sm-like domain, suggesting a role in RNA metabolism that was however not supported by experimental observations. Biochemical and biophysical evidence based on circular dichroism spectroscopy, light scattering, isothermal titration calorimetry and site-directed mutagenesis showed that RrCooT specifically binds a single Ni(ii) per dimer, with a dissociation constant of 9 nM, through the pair of Cys2, highly conserved residues, located at the dimer interface. Despite its role in the activation of RrCODH in vivo, CooT was thought to be a unique protein, found only in R. rubrum, with an unclear function. In this study, we extended the biological impact of CooT, establishing that this protein is a member of a novel Ni(ii)-binding protein family with 111 homologues, linked to anaerobic metabolism in bacteria and archaea, and in most cases to the presence of CODH. | |||
The CO dehydrogenase accessory protein CooT is a novel nickel-binding protein.,Timm J, Brochier-Armanet C, Perard J, Zambelli B, Ollagnier-de-Choudens S, Ciurli S, Cavazza C Metallomics. 2017 Apr 27. doi: 10.1039/c7mt00063d. PMID:28447092<ref>PMID:28447092</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5n76" style="background-color:#fffaf0;"></div> | ||
[[Category: Cavazza | == References == | ||
[[Category: Ciurli | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Rhodospirillum rubrum]] | ||
[[Category: Brochier-Armanet C]] | |||
[[Category: Cavazza C]] | |||
[[Category: Ciurli S]] | |||
[[Category: Ollagnier-de-Choudens S]] | |||
[[Category: Perard J]] | |||
[[Category: Timm J]] | |||
[[Category: Zambelli B]] | |||
