5n78: Difference between revisions
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New page: '''Unreleased structure''' The entry 5n78 is ON HOLD Authors: Lerche, M., Sandhu, H., Flockner, L., Hogbom, M., Rapp, M. Description: Crystal structure of the cytosolic domain of the C... |
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The | ==Crystal structure of the cytosolic domain of the CorA Mg2+ channel from Escherichia coli in complex with magnesium and cobalt hexammine== | ||
<StructureSection load='5n78' size='340' side='right'caption='[[5n78]], [[Resolution|resolution]] 2.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5n78]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N78 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n78 OCA], [https://pdbe.org/5n78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n78 RCSB], [https://www.ebi.ac.uk/pdbsum/5n78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n78 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CORA_ECOLI CORA_ECOLI] Mediates influx of magnesium ions. Can also mediate cobalt and manganese uptake (PubMed:780341). Alternates between open and closed states. Activated by low cytoplasmic Mg(2+) levels. Inactive when cytoplasmic Mg(2+) levels are high (By similarity).[UniProtKB:Q9WZ31]<ref>PMID:780341</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Structures of the Mg2+ bound (closed) and apo (open) states of CorA suggests that channel gating is accomplished by rigid-body motions between symmetric and asymmetric assemblies of the cytosolic portions of the five subunits in response to ligand (Mg2+) binding/unbinding at interfacial sites. Here, we structurally and biochemically characterize the isolated cytosolic domain from Escherichia coli CorA. The data reveal an Mg2+-ligand binding site located in a novel position between each of the five subunits and two Mg2+ ions trapped inside the pore. Soaking experiments show that cobalt hexammine outcompetes Mg2+ at the pore site closest to the membrane. This represents the first structural information of how an analog of hexa-hydrated Mg2+ (and competitive inhibitor of CorA) associates to the CorA pore. Biochemical data on the isolated cytoplasmic domain and full-length protein suggests that gating of the CorA channel is governed cooperatively. | |||
Structure and Cooperativity of the Cytosolic Domain of the CorA Mg2+ Channel from Escherichia coli.,Lerche M, Sandhu H, Flockner L, Hogbom M, Rapp M Structure. 2017 Aug 1;25(8):1175-1186.e4. doi: 10.1016/j.str.2017.05.024. Epub, 2017 Jun 29. PMID:28669631<ref>PMID:28669631</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5n78" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Lerche | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Escherichia coli]] | |||
[[Category: Large Structures]] | |||
[[Category: Flockner L]] | |||
[[Category: Hogbom M]] | |||
[[Category: Lerche M]] | |||
[[Category: Rapp M]] | |||
[[Category: Sandhu H]] | |||
Latest revision as of 12:31, 15 November 2023
Crystal structure of the cytosolic domain of the CorA Mg2+ channel from Escherichia coli in complex with magnesium and cobalt hexammine
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