5uv0: Difference between revisions
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New page: '''Unreleased structure''' The entry 5uv0 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures |
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==Crystal Structure of (+)-Limonene Synthase from Citrus sinensis== | |||
<StructureSection load='5uv0' size='340' side='right'caption='[[5uv0]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5uv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrus_sinensis Citrus sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UV0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uv0 OCA], [https://pdbe.org/5uv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uv0 RCSB], [https://www.ebi.ac.uk/pdbsum/5uv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uv0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RLC1_CITSI RLC1_CITSI] Catalyzes the conversion of geranyl diphosphate to (+)-(4R)-limonene (PubMed:28272875, PubMed:28272876). Produces exclusively the (+)-enantiomer (PubMed:28272875). Can use neryl diphosphate as substrate (PubMed:28272876). Has no activity with farnesyl diphosphate (PubMed:28272875).<ref>PMID:28272875</ref> <ref>PMID:28272876</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Terpenes make up the largest and most diverse class of natural compounds and have important commercial and medical applications. Limonene is a cyclic monoterpene (C10) present in nature as two enantiomers, (+) and (-), which are produced by different enzymes. The mechanism of production of the (-)-enantiomer has been studied in great detail, but to understand how enantiomeric selectivity is achieved in this class of enzymes, it is important to develop a thorough biochemical description of enzymes that generate (+)-limonene, as well. Here we report the first cloning and biochemical characterization of a (+)-limonene synthase from navel orange (Citrus sinensis). The enzyme obeys classical Michaelis-Menten kinetics and produces exclusively the (+)-enantiomer. We have determined the crystal structure of the apoprotein in an "open" conformation at 2.3 A resolution. Comparison with the structure of (-)-limonene synthase (Mentha spicata), which is representative of a fully closed conformation (Protein Data Bank entry 2ONG ), reveals that the short H-alpha1 helix moves nearly 5 A inward upon substrate binding, and a conserved Tyr flips to point its hydroxyl group into the active site. | |||
Functional and Structural Characterization of a (+)-Limonene Synthase from Citrus sinensis.,Morehouse BR, Kumar RP, Matos JO, Olsen SN, Entova S, Oprian DD Biochemistry. 2017 Mar 15. doi: 10.1021/acs.biochem.7b00143. PMID:28272875<ref>PMID:28272875</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5uv0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Citrus sinensis]] | |||
[[Category: Large Structures]] | |||
[[Category: Oprian DD]] | |||
[[Category: Prem Kumar R]] | |||
