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==X-ray crystal structure of Periplasmic Desferal binding protein FhuD from Vibrio cholerae==
==X-ray crystal structure of Periplasmic Desferal binding protein FhuD from Vibrio cholerae==
<StructureSection load='5ggy' size='340' side='right' caption='[[5ggy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='5ggy' size='340' side='right'caption='[[5ggy]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5ggy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GGY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5GGY FirstGlance]. <br>
<table><tr><td colspan='2'>[[5ggy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O395 Vibrio cholerae O395]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GGY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GGY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ggx|5ggx]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ggy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ggy OCA], [http://pdbe.org/5ggy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ggy RCSB], [http://www.ebi.ac.uk/pdbsum/5ggy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ggy ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ggy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ggy OCA], [https://pdbe.org/5ggy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ggy RCSB], [https://www.ebi.ac.uk/pdbsum/5ggy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ggy ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A0H3AJ03_VIBC3 A0A0H3AJ03_VIBC3]
The mechanism of haem transport across the inner membrane of pathogenic bacteria is currently insufficiently understood at the molecular level and no information is available for this process in Vibrio cholerae. To obtain structural insights into the periplasmic haem-binding protein HutB from V. cholerae (VcHutB), which is involved in haem transport through the HutBCD haem-transport system, at the atomic level, VcHutB was cloned, overexpressed and crystallized using 1.6 M ammonium sulfate as a precipitant at pH 7.0. X-ray diffraction data were collected to 2.4 A resolution on the RRCAT PX-BL-21 beamline at the Indus-2 synchrotron, Indore, India. The crystals belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 62.88, c = 135.8 A. Matthews coefficient calculations indicated the presence of one monomer in the asymmetric unit, with an approximate solvent content of 45.02%. Molecular-replacement calculations with Phaser confirmed the presence of a monomer in the asymmetric unit.
 
Purification, crystallization and preliminary X-ray analysis of the periplasmic haem-binding protein HutB from Vibrio cholerae.,Agarwal S, Biswas M, Dasgupta J Acta Crystallogr F Struct Biol Commun. 2015 Apr;71(Pt 4):401-4. doi:, 10.1107/S2053230X15003660. Epub 2015 Mar 20. PMID:25849499<ref>PMID:25849499</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ggy" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Agarwal, S]]
[[Category: Large Structures]]
[[Category: Dasgupta, J]]
[[Category: Vibrio cholerae O395]]
[[Category: Dey, S]]
[[Category: Agarwal S]]
[[Category: Ghosh, B]]
[[Category: Dasgupta J]]
[[Category: Abc transporter]]
[[Category: Dey S]]
[[Category: Apo-fhud]]
[[Category: Ghosh B]]
[[Category: Desferal]]
[[Category: Ferric siderophore binding protein]]
[[Category: Periplasmic]]
[[Category: Transport protein]]
[[Category: Vibrio cholerae]]
Retrieved from "https://proteopedia.org/5ggy"