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| [[Image:1tu8.gif|left|200px]]
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| {{Structure
| | ==STructure of Onchoverca volvulus Pi-class Glutathione S-transferase with its kompetitive inhibitor s-hexyl-GSH== |
| |PDB= 1tu8 |SIZE=350|CAPTION= <scene name='initialview01'>1tu8</scene>, resolution 1.80Å
| | <StructureSection load='1tu8' size='340' side='right'caption='[[1tu8]], [[Resolution|resolution]] 1.80Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene>
| | <table><tr><td colspan='2'>[[1tu8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Onchocerca_volvulus Onchocerca volvulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TU8 FirstGlance]. <br> |
| |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span>
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| |GENE= GST2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6282 Onchocerca volvulus])
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tu8 OCA], [https://pdbe.org/1tu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tu8 RCSB], [https://www.ebi.ac.uk/pdbsum/1tu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tu8 ProSAT]</span></td></tr> |
| |RELATEDENTRY=[[1tu7|1TU7]]
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tu8 OCA], [http://www.ebi.ac.uk/pdbsum/1tu8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tu8 RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/GSTP_ONCVO GSTP_ONCVO] Appears to play a central role in the parasite detoxification system. |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tu/1tu8_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tu8 ConSurf]. |
| | <div style="clear:both"></div> |
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| '''STructure of Onchoverca volvulus Pi-class Glutathione S-transferase with its kompetitive inhibitor s-hexyl-GSH'''
| | ==See Also== |
| | | *[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| Onchocerciasis is a debilitating parasitic disease caused by the filarial worm Onchocerca volvulus. Similar to other helminth parasites, O. volvulus is capable of evading the host's immune responses by a variety of defense mechanisms, including the detoxification activities of the glutathione S-transferases (GSTs). Additionally, in response to drug treatment, helminth GSTs are highly up-regulated, making them tempting targets both for chemotherapy and for vaccine development. We analyzed the three-dimensional x-ray structure of the major cytosolic GST from O. volvulus (Ov-GST2) in complex with its natural substrate glutathione and its competitive inhibitor S-hexylglutathione at 1.5 and 1.8 angstrom resolution, respectively. From the perspective of the biochemical classification, the Ov-GST2 seems to be related to pi-class GSTs. However, in comparison to other pi-class GSTs, in particular to the host's counterpart, the Ov-GST2 reveals significant and unusual differences in the sequence and overall structure. Major differences can be found in helix alpha-2, an important region for substrate recognition. Moreover, the binding site for the electrophilic co-substrate is spatially increased and more solvent-accessible. These structural alterations are responsible for different substrate specificities and will form the basis of parasite-specific structure-based drug design investigations.
| | [[Category: Large Structures]] |
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| ==About this Structure==
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| 1TU8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Onchocerca_volvulus Onchocerca volvulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TU8 OCA].
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| ==Reference==
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| Structure of the major cytosolic glutathione S-transferase from the parasitic nematode Onchocerca volvulus., Perbandt M, Hoppner J, Betzel C, Walter RD, Liebau E, J Biol Chem. 2005 Apr 1;280(13):12630-6. Epub 2005 Jan 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15640152 15640152]
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| [[Category: Glutathione transferase]] | |
| [[Category: Onchocerca volvulus]] | | [[Category: Onchocerca volvulus]] |
| [[Category: Single protein]]
| | [[Category: Perbandt M]] |
| [[Category: Perbandt, M.]] | |
| [[Category: transferase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:01:07 2008''
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