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[[Image:1u96.jpg|left|200px]]


{{Structure
==Solution Structure of Yeast Cox17 with Copper Bound==
|PDB= 1u96 |SIZE=350|CAPTION= <scene name='initialview01'>1u96</scene>
<StructureSection load='1u96' size='340' side='right'caption='[[1u96]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene>
<table><tr><td colspan='2'>[[1u96]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U96 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE= COX17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u96 OCA], [https://pdbe.org/1u96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u96 RCSB], [https://www.ebi.ac.uk/pdbsum/1u96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u96 ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u96 OCA], [http://www.ebi.ac.uk/pdbsum/1u96 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u96 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/COX17_YEAST COX17_YEAST] Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX.
 
== Evolutionary Conservation ==
'''Solution Structure of Yeast Cox17 with Copper Bound'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u9/1u96_consurf.spt"</scriptWhenChecked>
Cox17 is a 69-residue cysteine-rich, copper-binding protein that has been implicated in the delivery of copper to the Cu(A) and Cu(B) centers of cytochrome c oxidase via the copper-binding proteins Sco1 and Cox11, respectively. According to isothermal titration calorimetry experiments, fully reduced Cox17 binds one Cu(I) ion with a K(a) of (6.15 +/- 5.83) x 10(6) M(-1). The solution structures of both apo and Cu(I)-loaded Cox17 reveal two alpha helices preceded by an extensive, unstructured N-terminal region. This region is reminiscent of intrinsically unfolded proteins. The two structures are very similar overall with residues in the copper-binding region becoming more ordered in Cu(I)-loaded Cox17. Based on the NMR data, the Cu(I) ion has been modeled as two-coordinate with ligation by conserved residues Cys(23) and Cys(26). This site is similar to those observed for the Atx1 family of copper chaperones and is consistent with reported mutagenesis studies. A number of conserved, positively charged residues may interact with complementary surfaces on Sco1 and Cox11, facilitating docking and copper transfer. Taken together, these data suggest that Cox17 is not only well suited to a copper chaperone function but is specifically designed to interact with two different target proteins.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1U96 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U96 OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u96 ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Yeast cox17 solution structure and Copper(I) binding., Abajian C, Yatsunyk LA, Ramirez BE, Rosenzweig AC, J Biol Chem. 2004 Dec 17;279(51):53584-92. Epub 2004 Oct 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15465825 15465825]
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Abajian C]]
[[Category: Abajian, C.]]
[[Category: Ramirez BE]]
[[Category: Ramirez, B E.]]
[[Category: Rosenzweig AC]]
[[Category: Rosenzweig, A C.]]
[[Category: Yatsunyk LA]]
[[Category: Yatsunyk, L A.]]
[[Category: cytochrome c oxidase]]
[[Category: metallochaperone]]
[[Category: two alpha-helice]]
[[Category: unstructured n-terminus]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:09 2008''

Latest revision as of 06:35, 1 May 2024

Solution Structure of Yeast Cox17 with Copper Bound

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